【作者】 吴廷昌； 骆剑； 黄飞； 佘长宁； 陈猛猛； 陈国华； 黄文； 刘扬潮；

【Author】 Wu ting chang 1 ** , luo jian 1*** , huang fei 1 , se chang ning 1 , chen mengmeng 1 , chen guo hua 1*** , huang wen 2 , liu yang cao 3 (1.College of Ocean,Hainan University ; Key Laboratory of Tropic Biological Resources of Ministry of Education ,Hainan University,Haikou 570228,China;2. Institute of Aquatic Economic Animals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, school of Life Sciences,Sun Yat-sen University,Guangzhou 510275,China; 3.Hainan QingLi Aquatic breeding Corporation Ltd, Lingshui 572400,China)

【机构】 海南大学海洋学院海南大学热带生物资源教育部重点实验室； 中山大学水生经济动物研究所暨广东省水生经济动物良种繁育重点实验室； 海南青利水产繁殖有限公司；

【摘要】 将鞍带石斑鱼精子遗传物质进行紫外线照射灭活,灭活后的精子与斜带石斑鱼卵子混合授精,经低温冷休克处理加倍,静水培育孵化出膜。实验结果显示:鞍带石斑鱼精子在适宜的紫外处理剂量下,精子处理各实验组受精率和成活率均表现出了明显的Hertwig效应,未冷休克组显示出较高的受精率83.5%和单倍体率80%;在卵子受精后6min进行冷休克处理,水温在4-6℃的条件下,可以获得40%的受精率和29.7%的成活率。Duncan多重比较结果显示冷休克处理水温组和持续时间组在受精率方面差别不显著,而在孵化率方面持续时间组差异极显著(p=0.002),水温处理组差异并不显著(p=0.396),可能与卵子分裂受冷休克影响较小而持续发育受影响较大有关。更多还原

【Abstract】 The gynogenetic diploids of orange-spot grouper were induced by fertilization of mature eggs of orange-spot grouper with ultraviolet(UV)-irradiated sperm of giant grouper combined with cold shock. The result showed that the fertilization rate and hatchability present typical Herwig effect when the giant grouper sperm were treated in the appropriate intensity,in the contion of that ,the haploid control had a higher fertillization 83.5% and haploid rate 80%. At the condition of tempeture 4-6℃, the initiation time of 6 min after fertillization , it could get fertillization 40% and hatchability 29.7%.Duncan multiple comparison results indicated that the cold shock treatment water temperature group and duration of group difference in the fertilization rate was not significant.Only the hatchability in duration group differences was extremely significant (p = 0.002), difference between water temperature treatment group was not significant (p = 0.396).t may be relevant the comprehensive effect of temperature and duration time.更多还原