【作者】 王青林； 董云伟； 于姗姗； 董双林； 王芳；

【Author】 Wang Qing-Lin[1],Dong Yun-Wei[2],Yu Shan-Shan[1],Dong Shuang-Lin[1],Wang Fang[1] ([1]The Key Laboratory of Mariculture,Ministry of Education,Fisheries College,Ocean University of China;[2]College of Oceanography,Xiamen University)

【机构】 中国海洋大学海水养殖教育部重点实验室； 厦门大学海洋与环境学院；

【摘要】 本研究探讨了不同的饲养温度(16、21和26℃)对刺参(Apostichopus japonicus)生长及热耐受性的影响。实验持续40天,期间进行刺参生长、代谢、摄食以及能量收支的测定。结果表明,16℃下饲养的刺参体重(末体重11.96±0.35 g)明显重于21℃(10.33±0.41 g)和26℃(8.31±0.19 g)下的刺参(P<0.05),并且16℃下的刺参其生长能要明显高于其它两组(P<0.05)。在16、21和26℃下饲养的刺参的热临界极值分别为33.1、34.1和36.6℃,并且26℃饲养的刺参热休克蛋白基因表达量明显高于其它两组。这一结果表明:温度驯化能改变刺参的热耐受性,并且能通过临界热极值和热休克蛋白基因表达这两种指标进行反映。更多还原

【Abstract】 Effects of different rearing temperatures(16,21 and 26℃) on growth,metabolic performance and thermal tolerance of juvenile sea cucumber Apostichopus japonicus(initial body weight 7.72±0.96 g,mean±SD) were investigated in the present study.During the 40-day experiment,growth,metabolic performance,food intake and energy budget at different reared temperatures were determined.Sea cucumbers rearing at 16℃obtained better growth(final body weight 11.96±0.35 g) than those reared at 21(10.33±0.41 g) and 26℃(8.31±0.19 g)(P<0.05), and more energy was allocated for growth at 16℃(162.73±11.85 J g-1 d-1) than those at 21(79.61±6.76 J g-1 d-1) and 26℃(27.07±4.30 J g-1 d-1)(P<0.05).Critical thermal maxima(CTmax) values of juvenile sea cucumbers reared at 16,21 and 26℃were 33.1,34.1 and 36.6℃,respectively,and the upregulation of hsps in sea cucumbers reared at 26℃was higher than those acclimated at lower temperatures(16 and 21℃),indicating that temperature acclimation could change the thermal tolerance of the sea cucumber,and CTmax and hsps were sensitive indicators of the sea cucumber’s thermal tolerance.更多还原