【作者】 林师； 王国栋； 张丽莉； 邹志华； 王艺磊；

【Author】 Shi Lin,Guodong Wang,Lili Zhang,Zou Zhihua,Yilei Wang (Fisheries College,Jimei University,Xiamen,Fujian,China,361021)

【机构】 集美大学水产学院；

【摘要】 Perluicn属于C型凝集素家族,是软体动物特有的一种凝集素。通过检索课题组自建的杂色鲍EST数据库,筛选出perlucin的6条EST,分属不同的亚型。利用SMART RACE技术获得这6条perluicn基因的全长cDNA序列。根据同其它物种序列的相似性分别命名为perlucin 1、perlucin2、perlucin4、perlucin5、perlucin6和perlucin8。并采用Real time PCR和半定量PCR分析这些基因在杂色鲍组织中的表达情况。其中perlucin8比较特殊,具有非常短的ORF仅编码40个氨基酸,等电点约为10.85,偏碱性。其它5条perlucin基因的全长在550bp-760bp之间;开放阅读框编码约160个氨基酸,含有2个二硫键、1-2个糖基化位点和10-14个磷酸化位点;预测蛋白质分子量范围在18-20kDa,等电点范围在4.5-6.0之间。perlucinl基因和perlucin4基因在肝胰腺中特异表达。perlucin5基因在外套膜中的表达量最高,鳃中表达量最低。更多还原

【Abstract】 Perlucin belongs to C-lectin family,is speciestypical lectin of mollusk.Six ESTs from H.diversicolor cDNA library was found to be homologous with different perlucin substypes. Subsequently,their full-length cDNAs were obtained by SMART RACE.The six full-length cDNAs were named perlucinl,perlucin2,perlucin4,perlucin5,perlucin6 and perlucin8,according to similarity to other species.Quantitative real-time PCR and semi-quantitative PCR werr employed to investigate the tissue distribution of perlucins mRNA.Perlucin8 has a very short ORF,coding 40 amino acids,with an estimated pI of 10.85.The full length of the other 5 perlucins is 550bp-760bp,encoding about 160 amino acids,with 2 disulfide bonds,1-2 glycosylation sites and 10-14 phosphorylation sites.The predicted molecular weight is 18-20kDa with pI of 4.5-6.0.Perlucinl and perlucin4 express only hepatopancreas.Perlucin5 could be detected in all examined tissues,with the highest espression level in mantle and the lowest in gill.更多还原