【作者】 吴兵； 罗宇良； 王敏； 曹胜波； 王卫民； 陈龙； 黄锋涛； 刘学芹；

【Author】 Wu bing~1 Luo yu-liang~1 Wang min~1 Cao sheng-bo~2 Wang wei-ming~1 Chen long~2 Huang feng-tao~1 Liu xue-qin~1(*) (1 College of Fisheries,Huazhong Agricultural University,Wuhan 430070,China 2 College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China)

【机构】 华中农业大学水产学院； 华中农业大学动物医学院；

【摘要】 本实验将纯化好的,从斑点叉尾鮰卵巢细胞增殖的斑点叉尾鮰病毒免疫四周龄BABL/C雌鼠,获得斑点叉尾鮰病毒粒子多克隆抗体,通过酶联免疫吸附试验检测其抗体效价,同时利用间接免疫荧光实验和血清中和试验检测所制备多克隆抗体的生物活性。试验结果表明,实验获得了较高浓度的病毒悬液,制备得到的多克隆抗体ELISA效价为1:25600,间接免疫荧光实验证明抗体特异性良好,同时血清中和试验证明多克隆抗体能够很好地阻断病毒对细胞的感染,中和效价为1:256。更多还原

【Abstract】 Purified channel catfish virus was reproducted in the channel catfish ovary and then used to immunize the female BALB/C mice to gain polyclonal antibody.ELISA was used to detect the titer and the bioactivity was identified by indirect immunofluorescent assay and serum neutralization tests.The titer of the polyclonal antibody is 1:25600 by ELISA.The polyclonal antibody shows high Specificity at the indirect immunofluorescent assay test.The polyclonal antibody can stop the virus from infecting the cell efficiently with the neutralisation titer 1:256.更多还原