【作者】 马欢； 刘伟伟； 王秀菊； 刘士清； 李建昌； 张无敌；

【Author】 MA Huan~(1,3)LIU Wei-Wei~(2,3)WANG Xiu-Ju~3 LIU Shi-Qing~(*3) LI Jian-Chang~3 ZHANG Wu-Di~3 1.Key Laboratory of Ion Beam Bioengineering,Institute of Plasma Physics, Chinese Academy of Sciences Hefei 230031 PR China 2.Technology School of Anhui Agricultural University Hefei 230036 P.R.China 3.Provincial Key Laboratory of Rural Energy Engineering Yannan Normal University,Kunming 650092

【机构】 中国科学院等离子体物理研究所离子束生物工程重点实验室； 云南师范大学省农村能源工程重点实验室； 安徽农业大学工学院；

【摘要】 以（H₂N）₂CO、[SO₄]^2-、Cl^-、[NO₃]^-、[Mo₂O₂₄]^6-、[C₂O₄]^2-、[C₆H₅O₇]^3-6种不同的阴离子和尿素对生物质发酵产氢的影响,分析得知H₂与CO₂和总产气量存在显著的正比关系;与糖利用率无直接关系。经统计分析得到了以混合菌群为接种物下,对生物质发酵产氢具有促进作用的阴离子组成为:H₂NCONH₂0g/L,[SO₄]^2-1.5g/L,Cl^-0g/L,[NO₃]^- 0g/L,[Mo₇O₂₄]^6-0.1g/L,[C₂O₄]^2-1g/L,[C₆H₅O₇]^3-3-5g/L。以PDB液为基质发酵原料验证发酵气体产生比对照组提前了7h,而且H₂最高含量达60.7%,平均含量50.2%,分别比对照组提高了21.4%和24.1%;平均产氢速率由对照组的36.79mL/L.h提高到96.25mL/L.h;氢气产率由对照组的0.76mol/mol提高到1.17mol/mol。研究证明:尿素对H₂的产生有极显著的抑制作用,[Mo₇O₂₄]^6-则相反。揭示了[C₂O₄]^2--[C₆H₅O₇]^3-阴离子对组合的交互作用改善发酵产氢条件,并从理论上进行了分析探讨。更多还原

【Abstract】 With L₂₇(3¹³)orthogonal test,the effect of、[SO₄]^2-、Cl^-、[NO₃]^-、[Mo₇O₂₄]^6-、[C₂ O₄]^2-、[C₆H₅O₇]^3-six different negative ions and（H₂N）₂CO on hydrogen production by microorganism flora was studied.The result showed that hydrogen had prominent direct ratio relationship with carbon dioxide and the total gas yield while without glucose utilization.By statistics method,the promoting component to hydrogen production was obtained,which consists of H₂NCONH₂ 0g/L,SO₄₂- 1.5g/L,Cl^-0g/L,NO₃₊0g/L,(Mo₇O₂₄)^6-0.1g/L,C₂O₄₂-1g/L,（C₆H₅O₇）^3-5g/L,whereas it is demonstrated that with the promoting component the maximal hydrogen content and the average hydrogen content from PDB were respectively raised 21.4%and 24.1%,and hydrogen yield compared to antitheses was raised from 0.76 mol/mol to 1.17 mol/mol It was suggested that carbamide prominently in- hibited the production of hydrogen contrary to[Mo₇O₂₄]^6-and the couple of[C₂O₄]^2--[C₆H₅O₇]^3- negative ions was able to optimize the condition of hydrogen production,which was analyzed theoretically.更多还原