【作者】 许民辉； 周椿； 沈光建； 徐伦山； 易良；

【Author】 Xu minhui1 Cheng yuan1 Zhou Chun2 Shen guangjian2 Xu lunshan2 Yi liang2 1 Department of Neurosurgery,The Second Affiliated Hospital,Chongqing Medical University,Chongqing,China;2 Department of Neurosurgery,Daping Hospital,Third Military Medical University,Chongqing,China

【机构】 第三军医大学附属大坪医院神经外科；

【摘要】 目的:探讨PTEN对大鼠原代海马神经元牵张损伤后AMPA受体GluR2亚单位定位表达的调节作用。方法:选用新生SD大鼠培养原代海马神经元,建立神经元牵张损伤模型,利用RNAi慢病毒载体敲减神经元中PTEN基因的表达。采用Tunel染色评估神经元牵张各时相点的损伤情况。采用Western-blot检测牵张各时相点AMPA受体GluR2亚单位在细胞中的表达定位变化。结果:慢病毒介导的RNAi能有效下调PTEN基因的表达。PTEN基因表达下调后,神经元牵张各时相点细胞总蛋白和膜蛋白中GluR2亚单位表达无明显变化,凋亡细胞比例明显下降。结论:海马神经元受到机械性牵张损伤后,下调PTEN的表达能够有效地阻止AMPA受体GluR2亚单位在细胞膜上定位的降低,降低凋亡细胞比例。PTEN对AMAP受体的调节作用可能为预防和治疗脑创伤提供一条新途径。更多还原

【Abstract】 Objective:To explore the effect of PTEN in the regulation on localization of AMPA recepter subunit GluR2 in rat hippocampal neuron after stretch Injury.Methods:Cultured the primary rat hippocampal neuron from neonate rats and established the model of stretch injury.Utilized RNAi-lentvirus to inhibit the expression of PTEN gene.Used Tunel dyeing to assess injury of neurons and used Western-blot to investigate localization of AMPA recepter subunit GluR2 in rat hippocampal neuron after stretch Injury.Results:RNAi-lentvirus can downregulated expression of PTEN gene effectively.When decresase expression of PTEN,the proportion of the apoptotic cells decreases evidently and there is no obvious downregulation of GluR2 subunits content in total protein and membrane protein.Conclusion:Downregulation of PTEN can inbibit the decrease of AMPA recepter GluR2 subunit in cell membrane of neuron after stretch injury,prevent the apoptosis of neuron.The function of PTEN to AMPA recepter may provides a new way for the prevention and treatment of traumatic brain injury.更多还原