【作者】 严作廷； 米宝明； 宗瑞谦； 祝秉东； 王桂荣； 李世宏； 王东升；

【Author】 Yan Zuoting;Mibaoming;Zongruiqian;Zhubingdong;Wangguirong;Lishihong;Wangdongsheng;Lanzhou Institute of Animal and Veterinary Pharmaceutics Sciences,Chinese Academy of Agricultural Sciences;College of Veterinary Medicine,Gansu Agricultural University;Basic Medical College of Lanzhou University;

【机构】 中国农业科学院兰州畜牧与兽药研究所； 甘肃农业大学动物医学院； 兰州大学基础医学院；

【摘要】 研究目的:为研发奶牛抗菌肽生物制剂,防治奶牛子宫内膜炎疾病。研究方法:采用酸性尿素聚丙烯酰胺凝胶电泳琼脂糖弥散法检测子宫内膜粘液酸溶性提取物抗菌活性:应用琼脂糖电泳及水平电泳洗脱分离相应的抗菌条带,HPLC进一步分离纯化,琼脂糖弥散法检测纯化分子抗菌活性,Tricinc-SDS-PAG电泳检测其分子量。研究结果:从奶牛子宫内膜酸溶性提取物中纯化出一分子量约为14KD左右的抗菌多肽,对大肠杆(E-coli.BL21)和金黄色葡萄球菌26003具有抗菌活性。研究结论:可能是一种新的子宫内膜分泌的抗菌多肽,参与了奶牛子宫的天然防御机制。更多还原

【Abstract】 Objective:To research and development biological agents of Dairy Cow antimicrobial peptides,Prevention and treatment of endometritis cow disease.Methods:acid-soluble extract of cow uterus mucus was obtained and antibacterial activity was detected by gel overlay technique,Then it was further isolated and purified by HPLC and analyzed antibacterial activity by agarose radial diffusion assay.The molecular weight was determined by Tricine-SDS-PAGE.Results:It was able to effectively kill E.coliBL21 and the Staphylococcus bacteria 26003 its molecular weight was about 14KDa.Conclusion:It may be a new antibacterial polypeptide from cow uterus mucus and take part in natural immunology defense in uterus mucus.更多还原