IL-17A与喉鳞状细胞癌的相关性研究

【作者】 孟粹达； 安立峰； 姜晓丹； 朱冬冬； 董震； 李琳； 孔红；

【机构】 吉林大学中日联谊医院耳鼻咽喉头颈外科；

【摘要】 目的检测白介素-17(IL-17A)在喉鳞状细胞癌组织、喉炎症组织和喉修补/正常黏膜中的表达及外周血中的表达。探讨IL-17 A在喉鳞状细胞癌发生发展过程中的作用和意义。方法 32例喉鳞状细胞癌组织,20例慢性肥厚性喉炎组织及10例行喉修补术的喉粘膜或喉开放性外伤喉修补粘膜作为正常对照。留取喉癌及喉炎患者的血清及20例正常血清对照。HE染色用于常规组织病理分型及评价粘膜炎症程度,免疫组织化学染色用于观察IL-17A、IL-17RD,CD68,CD34表达水平及细胞定位,免疫组织化学双重染色用于观察和测定IL-17A与巨噬细胞的表达水平。ELISA方法检测外周血中IL-17A水平。结果①32例喉癌组织中IL-17A阳性细胞数较正常对照组织中明显增多,两者比较差异均有显著性(P<0.05)。②IL-17及CD68双重免疫组化染色证明IL-17A主要定位于巨噬细胞。③IL-17A的高表达与肿瘤的分化程度相关。④喉鳞状细胞癌组织中IL-17A的表达与炎症程度及微血管密度成正相关。⑤三组外周血中IL-17A蛋白水平相比差异无统计学意义(P>0.05)。结论①IL-17A介导的炎症可能在喉鳞状细胞癌的发生发展过程中通过影响巨噬细胞的功能及相关细胞因子实现的。②IL-17A的表达与喉鳞状细胞癌的分化、微血管密度及炎症浸润呈正相关。③IL-17A在喉鳞状细胞癌的浸润转移中发挥一定的作用。更多还原

【Abstract】 objective To test the expression of interleukin 17A(IL-17 A) among squamous cell carcinoma of larynx,laryngitis and repair tissue/normal mucosa of laryngeal.And the expression of IL-17A in peripheral blood of patients.Explore the role and meaning of IL-17 A in the development process of squamous cell carcinoma of larynx.Method 32 cases of squamous cell carcinoma of larynx,20 cases of chronic hypertrophic laryngitis organization and 10 cases of the repair mucosal of laryngeal carcinoma patients or the open injury of throat repair mucosa as normal control.conserve the serum of Laryngeal cancer patients,and laryngitis patients and 20 cases of health serum do normal controls.HE dyed used for routine organization pathology classification and evaluation mucosal inflammation degree, immunohistochemical stain used to observe IL-17 A,IL-17 RD,CD68 CD34 expression level,and cell positioning,immunohistochemical double stain used to observe and determination of IL-17 A and macrophages expression level.ELISA method detection the expression or the protein level of IL-17 A of three groups’ peripheral blood.Results①the IL-17A positive cells number of 32 cases of laryngeal cancer increased than normal control group and comparative differences are significant(P<0.05).②IL-17 and CD68 double immunohistochemical stains prove IL-17 A main role in macrophages.③IL-17A high express related of the differentiation of laryngeal carcinoma.④the degree with the high expression of IL-17A of throat squamous cell carcinoma and inflammation and capillaries density are positive correlated.⑤The level of IL-17A in the three groups’ peripheral blood is no statistically difference significant(P > 0.05). Conclusion①with the development of squamous cell carcinoma of larynx,the inflamation happen in the process of the IL-17 A mediated may be influenced by macrophage cell function and related cell factors to realize.②In the squamous cell carcinoma of larynx,expression of IL-17 A and the differentiation and microvascular density and inflammatory infiltration were positively correlated.③IL-17A play a certain role in the metastasis of squamous cell earcinoma of larynx.更多还原