【作者】 赵立平； 朱玛； 姜广路； 于霞； 黄海荣；

【Author】 1 Beijing Tuberculosis & Thoracic Tumor Institute,Beijing,China 101149 2 Chengdu Tuberculosis Hospital,Chengdu,Sichuan 611933 hao Liping~1,Zhu Ma~2,Jiang Guanglu~1,Yu Xia~1,Huang Hairong~1

【机构】 北京市结核病胸部肿瘤研究所； 成都市结核病防治院；

【摘要】 目的比较分析分枝杆菌罗氏培养时分别采用简单法和离心法时结果的差异;研究离心法罗氏培养时在标本处理过程中不同离心速度和不同离心时间对培养结果的影响。方法选取149份临床诊断为结核病患者的痰标本,其中涂片阳性的痰标本95份,涂片阴性的痰标本54份。同时应用简单法和离心法对158份痰标本进行罗氏培养;离心法培养时分别采用5种不同的离心条件:5000g/15min;3000g/15min;2000g/15min;3000g/25min;3000g/8min。结果在选取的149份痰标本中,共有122份标本培养阳性,其中94份来自涂阳痰标本,29份来自涂阴痰标本;总体污染率简单法为4.02%(6/149),离心法为5.36%(8/149)。在所有的122份培养阳性的标本中,排除污染,简单法的阳性率为100%(121/121),离心法的阳性率为99.16%(119/120);简单法的平均初生长时间18.67±6.14d,离心法的平均初生长时间为20.41±5.74d,简单法较离心法的平均初生长时间短1.74天(P<0.05)。在119份离心法培养阳性的标本中,5000rpm/15min、3000rpm/15min、2000rpm/15min、3000rpm/25min、3000rpm/8min等5种不同离心条件下培养阳性率对分别为100%,100%,96.63%,99.15%和94.95%。结论罗氏培养过程中,简单法的阳性率与离心法接近,但由于简单法操作简单、污染率较低,初生长时间较短,因而可能更符合临床的需要;应用离心法进行罗氏培养时,离心力或离心时间的变化可能会影响培养的阳性率。更多还原

【Abstract】 Object To analyze the outcome difference of LJ medium culture when using simple method and centrifuge method simultaneously,and also assess the influence of centrifuge force and centrifuge duration time on culture outcome when using centrifuge method.Method Collected 149 sputum specimens from clinically diagnosed tuberculosis patients,among them,95 was smear positive,54 was smear negative.Performed LJ medium culture for each specimen by using simple method and centrifuge method at the same time.For the centrifuge method,tried following centrifuge status simultaneously:5000g/15min;3000g/15min;2000g/15min; 3000g/25min;3000g/8min.Results 122 specimens were culture positive among all 149 sputum specimens, among them,94 originated from smear positive sputa,29 originated from smear negative sputa;the contamination rate for simple method was 4.02%(6/149),for centrifuge method was 5.36%(8/149.Among all 122 culture positive specimens,excluding the contaminated samples,the positive rate for simple method was 100%(121/121),and for centrifuge method was 99.16%(119/120);The average turn around time for simple method was 18.67±6.14 day,and 20.41±5.74 day for centrifuge method,simple method had 1.74 day shorter turn around time than centrifuge method(P<0.05).Among 119 culture positive specimen by centrifuge method,The positive rate had minor difference considering different centrifuge status:100%for 5000rpm/15min,100%3000rpm/15min, 96.63%for 2000rpm/15min、99.15%for 3000rpm/25min、94.95%for 3000rpm/8min..Conclusion Simple method LJ medium culture has similar sensitivity with centrifuge method,but since it is priority to centrifuge method concerning simplicity,lower contamination rate and shorter turn around time,so it maybe more feasible to use widely;centrifuge force and centrifuge duration time is a influencing factor for culture positive rate when using centrifuge method..更多还原