Fertility conversion related proteins in Temperature Sensitive Genic Male Sterile Line K121S of Brassica juncea

【作者】 刘岱； 张传利； 彭少丹； 林良斌；

【机构】 昆明医科大学图书馆； 云南农业大学热带作物学院； 云南农业大学农科基础课程实验教学学中心； 云南农业大学农学与生物技术学院；

【摘要】 The present study was conducted to study fertility conversion related proteins in Brassicajuncea and to reveal the molecular mechanism of temperature sensitive male sterility. Weused IEF / SDS-PAGE and PDQuest analysis software technology to study the proteins atthe three nuclear flower bud stage in temperature sensitive genic male sterile line(TGMS)K121S under infertile and fertile temperatures. Proteomic analysis of plants grown at differenttemperatures revealed that a total of 2377 proteins spots were detected at fertiletemperature and 2555 spots at the infertility temperature. A total of 1522 protein spotswere common in plants grown at the two different temperatures and 855 and 1033 spotswere specific to fertility and infertility temperatures respectively. Moreover, 1522 commonproteins spots were observed in both plant samples. Mass spectrometry analysis for 69 protein points showed that a majority of these proteins(54.4%) are associated with energymetabolism. The remainder was identified to be related to growth and development, defense,protein synthesis, transcription factors and signal transduction, cytoplasmic movementand cell division, protein folding, oxygen radical scavenging related proteins etc. Wepropose that the mechanisms of K121 S fertility conversion can be attributed to the abnormalsplicing of material and energy metabolism related proteins at the mRNA level causingabnormal function at infertile temperatures. These proteins include ribulose bisphosphatecarboxylase, ribulose phosphate epimerase-3. Moreover, the transcription of actin, myosinand APC cofactor genes were probably affected by the temperature sensitive BTB/POZ regulatory domain, which caused male sterility.更多还原

【Abstract】 The present study was conducted to study fertility conversion related proteins in Brassicajuncea and to reveal the molecular mechanism of temperature sensitive male sterility. Weused IEF / SDS-PAGE and PDQuest analysis software technology to study the proteins atthe three nuclear flower bud stage in temperature sensitive genic male sterile line(TGMS)K121S under infertile and fertile temperatures. Proteomic analysis of plants grown at differenttemperatures revealed that a total of 2377 proteins spots were detected at fertiletemperature and 2555 spots at the infertility temperature. A total of 1522 protein spotswere common in plants grown at the two different temperatures and 855 and 1033 spotswere specific to fertility and infertility temperatures respectively. Moreover, 1522 commonproteins spots were observed in both plant samples. Mass spectrometry analysis for 69 protein points showed that a majority of these proteins(54.4%) are associated with energymetabolism. The remainder was identified to be related to growth and development, defense,protein synthesis, transcription factors and signal transduction, cytoplasmic movementand cell division, protein folding, oxygen radical scavenging related proteins etc. Wepropose that the mechanisms of K121 S fertility conversion can be attributed to the abnormalsplicing of material and energy metabolism related proteins at the mRNA level causingabnormal function at infertile temperatures. These proteins include ribulose bisphosphatecarboxylase, ribulose phosphate epimerase-3. Moreover, the transcription of actin, myosinand APC cofactor genes were probably affected by the temperature sensitive BTB/POZ regulatory domain, which caused male sterility.更多还原