Analysis of genetic diversity and relationship of Spinach by AFLP

【作者】 王文科；

【Author】 Wang Wen-Ke Wu Ya-Ni School of life scence normal university, linfen,shanxi,041004,chian.

【机构】 山西师大生命科学学院；

【摘要】 <正>Spinach belongs to Spinacia Linn of Chenopodiaceae,it was planted natively in Iran 2000 years ago.Spinach is cultivated widely all over the world and it is one of the most import-ant exported vegetables in our country.It has many merits such as extensive adaptability,endurable storage,long supplying stage and many cultivated mode.In addition, it has abundant vitamin C,carotene and ferrum.So it is very important to collect,evaluate and utilize the Spinach germplasm. 127 representative Spinach accessions were collected from different countries or regions in order to study on their genetic and relationships.In this study,genetic diversity were analysed by AFLP.The aim is that provided the theoretical basis with Spinach breeding.In this study,the key procedures of AFLP were optimized.A system suitable for the genetic diversity of Spinach AFLP was established.Using the optimized system,28 primer pairs of E+3/M+3 primer combination were screened out from 256 pairs.A total of 827 scorable bands were amplified,of which 276(33.37%) showed polymorphic.The highest polymorph-ic primer pair was E36M41,and its polymorphic rate was 62.72%.On the contrary,E41M32 primer pair generated the lowest polymorphic bands,which polymorphic percentage was 14.71%.The results indicated that primer selection was important for overall understand-ing of Spinach genetic diversity.UPGMA clustering method was carried out by NTSYS analysis software.The genetic simil-arity coefficient(GS) of 127 materials ranged between 0.65 and 0.87.Based on material similarity coefficient,the tested materials were clustered.127 accessions could be tax-onomied into three groups in the genetic similarity coefficient of 0.71.The first group is mainly the western varieties,the second group is mostly the Chinese varieties,and the third group is the Japanese varieties.Among the three groups,the relationship between Chinese and Japanese varieties was closer,the western varieties was next.Chinese varieties included plentiful germplasm.The genetic diversity of Spinach proved that it had broader genetic background from DNA level.It is beneficial to the collection,conservation and util-ization of Spinach germplasm.更多还原

【Abstract】 Spinach belongs to Spinacia Linn of Chenopodiaceae,it was planted natively in Iran 2000 years ago.Spinach is cultivated widely all over the world and it is one of the most import-ant exported vegetables in our country.It has many merits such as extensive adaptability,endurable storage,long supplying stage and many cultivated mode.In addition, it has abundant vitamin C,carotene and ferrum.So it is very important to collect,evaluate and utilize the Spinach germplasm. 127 representative Spinach accessions were collected from different countries or regions in order to study on their genetic and relationships.In this study,genetic diversity were analysed by AFLP.The aim is that provided the theoretical basis with Spinach breeding.In this study,the key procedures of AFLP were optimized.A system suitable for the genetic diversity of Spinach AFLP was established.Using the optimized system,28 primer pairs of E+3/M+3 primer combination were screened out from 256 pairs.A total of 827 scorable bands were amplified,of which 276(33.37%) showed polymorphic.The highest polymorph-ic primer pair was E36M41,and its polymorphic rate was 62.72%.On the contrary,E41M32 primer pair generated the lowest polymorphic bands,which polymorphic percentage was 14.71%.The results indicated that primer selection was important for overall understand-ing of Spinach genetic diversity.UPGMA clustering method was carried out by NTSYS analysis software.The genetic simil-arity coefficient(GS) of 127 materials ranged between 0.65 and 0.87.Based on material similarity coefficient,the tested materials were clustered.127 accessions could be tax-onomied into three groups in the genetic similarity coefficient of 0.71.The first group is mainly the western varieties,the second group is mostly the Chinese varieties,and the third group is the Japanese varieties.Among the three groups,the relationship between Chinese and Japanese varieties was closer,the western varieties was next.Chinese varieties included plentiful germplasm.The genetic diversity of Spinach proved that it had broader genetic background from DNA level.It is beneficial to the collection,conservation and util-ization of Spinach germplasm.更多还原