【作者】 戴方伟； 周莎桑； 宋晓明； 卢领群； 柯贤福； 郭红刚； 李长龙； 贺争鸣； 陈振文； 萨晓婴；

【Author】 DAI Fang-wei~1,ZHOU Sha-sang~1,SONG Xiao-ming~1,LU Ling-qun~1,KE Xian-fu~1,GUO Hong-gang~1,LI Chang-long~1,HE Zheng-ming~2,CHEN Zhen-wen~3,SA Xiao-yin~1 (1 Zhejiang Academy of Medical Sciences,Zhejiang Center of Laboratory Animals.Hangzhou 310013 2 Institute of Laboratory Animal Resources,National Institutes for food and Drug control.Beijing 100050 3 Department of Laboratory Animal sciences,Capital University of Medical Sciences.Beijing 100069 )

【机构】 浙江省医学科学院,浙江省实验动物中心； 中国食品药品检定研究院实验动物资源研究所； 首都医科大学基础医学院实验动物学系；

【摘要】 目的比较长爪沙鼠生物净化前后微生物携带情况。方法采集生物净化前后长爪沙鼠的气管、回盲部样本,经血平皿培养后,分离菌落进行纯培养,利用细菌鉴定仪进行菌种鉴定;气管经研磨后,转入选择性培养基鉴别培养;分离血清进行泰泽病原体抗体检测。结果生物净化后F1代、F2代和F3代长爪沙鼠泰泽病原体血清抗体检测均为阴性,生物净化后的其他微生物和寄生虫普查结果也表明,各项检测中均未发现清洁级啮齿类动物中需排除的病原微生物。结论本研究证实剖腹产和代乳技术能有效降低长爪沙鼠携带的微生物和寄生虫。更多还原

【Abstract】 Objective To compare microbial carrying between pre- and post-cleaning.Methods Microbial samples were taken from trachea and ileocecal part.After cultivated on Columbia blood agar,colonies were differentiated automatically. Trachea was polished and cultured in selective broth for mycoplasma detection.Serum samples were used for Tyzzer’s antibody detection.Results None positive sample of Tyzzer’s antibody response was detected in colonies post-cleaning. Other pathogenic microbiology and parasites detection also showed that no serious pathogen was found in the biological cleaning gerbils.Conclusion It could be concluded that the caesarean section and the milk replacer technology can effectively reduce the gerbil carrying microorganisms and parasites.更多还原