【作者】 刘荣辉； 李华； 左启祯； 于辉； 赵红娟；

【Author】 LIU Rong-hui~(1,2,3),LI Hua~1,ZUO Qi-zhen~1,YU Hui~1,ZHAO Hong-juan~(1,2) (1.Department of Animal Science,Foshan University,Nanhai 528231,China;2.College of Animal Science and Technology, Sichuan Agriculture University,Ya’an 625014,China;3.The Kaixian Bureau of Husbandry and Veterinary,Chongqing,400000, China)

【机构】 佛山科学技术学院动物科学系； 四川农业大学动物科技学院； 开县畜牧兽医局；

【摘要】 目的为研究明光小耳猪、滇南小耳猪、巴马香猪遗传多样性以及命名,本研究利用猪白细胞抗原复合物(SLA)区域内18个微卫星,探讨了3品种小型猪和云南野猪(S.s.silvianus)的观测杂合度(Ho)、期望杂合度(He)、多态信息含量(PIC)等遗传参数,且从单倍型分型、聚类分析以及主成分分析方面进一步探讨了它们之间的亲缘关系。结果表明:云南野猪多态性最高(PIC=0.694),巴马香猪最低(PIC=0.585),3品种小型猪均具有丰富的遗传多样性。在检测群体中SLAⅠ、Ⅱ区域分别有12种和14种单倍型,其中明光小耳猪、滇南小耳猪和云南野猪共享3个单倍型H3,H4,H03,巴马香猪仅与明光小耳猪共享单倍型H2。通过系统聚类和主成分分析发现,明光小耳猪、滇南小耳猪与云南野猪亲缘关系较近,巴马香猪与之关系较远。研究结果提示,明光小耳猪和滇南小耳猪具有相似的遗传背景,明光小耳猪可能是滇南小耳猪的一个品系,这为解决同种异名问题提供了分子证明。更多还原

【Abstract】 Objective To study the genetic polymorphism and nomenclature of Mingguang small-ear pig(MG),Diannan small-ear pig(DN) and Bama xiang pig(BM),the polymorphic parameters were calculated using eighteen microsatellites(MS) in swine leukocyte antigen complex(SLA).The genetic parameters,including the observed heterozygosity(Ho),expected heterozygosity(He) and the polymorphism information content(PIC),were studied in three breed miniature pigs and Yunnan wild boar(YWB,S.s.silvianus).The haplotypes,clustering and principal component(PC) analysis were further studied to elucidate the relationship in three breed miniature pigs.The results showed that the PIC of Yunnan wild boar was highest(0.694) versus Bama xiang pig was lowest(0.585),these miniature breed pigs were highly polymorphic.There were twelve and fourteen kinds of haplotypes in the regions of SLAⅠ,Ⅱ,respectively.Three shared haplotypes were found among Mingguang small-ear pig, Diannan small-ear pig and Yunnan wild boar,only one haplotype was detected between Bama xiang pig and Mingguang small-ear pig.Comparison with Bama xiang pig,the relationship was close in Mingguang small-ear pig,Diannan small-ear pig and Yunnan wild boar by the clustering and PC analysis.These results suggest that Mingguang small-ear pig can belong to Diannan small-ear pig,because they are shared similar genetic background,this study gives us a molecular evidence for the synonym issue.更多还原