【作者】 郑龙； 连伟光； 李建辉； 张焕铃； 王俊霞； 刘军须； 蔡月花； 刘福英；

【Author】 ZHENG Long,LIAN Wei-guang,LI Jian-hui,ZHANG Huan-ling,WANG Jun-xia,LIU Jun-xu,CAI Yue-hua,LIU Fu-ying (Hebei Key Laboratory of Experimental Animal,Hebei Medical University,Shijiazhuang 050017)

【机构】 河北医科大学河北省实验动物重点实验室；

【摘要】 目的通过喂养高脂饮食,建立近交系HFJ大鼠的脂肪肝和胰岛素抵抗动物模型。方法选择HFJ大鼠和Wistar大鼠,雌雄各半,随机分为模型组和正常组,模型组喂养高脂饮食,正常组喂养普通饮食,两组脂肪分别占摄人能量的44.2%和19.2%,共饲养12周。每周称体质量,测定血糖、甘油三酯、胆固醇、ALT、AST、HDLC、LDLC和血胰岛素水平。实验期末处死动物摘取肝脏并称质量,计算肝指数。鼠肝脏用10%甲醛固定,石蜡包埋,HE染色,光镜下评估肝脂肪变性和炎症活动情况。结果 HFJ和Wistar大鼠模型组肝细胞均呈现弥漫性脂肪变性,可见小叶内炎症细胞浸润。对照组肝脏无异常。模型组ALT、AST、肝指数、HOMA-IR指数均显著高于正常组,HFJ和Wistar品系间无显著差异;HFJ雄性大鼠模型组和正常组体重具有显著差异(p<0.01),而Wistar雄性大鼠模型组与正常组间无显著差异(p>0.05);HFJ雄性大鼠TG和TC含量显著高于Wistar大鼠;模型组HFJ和Wistar雌性大鼠TC含量显著高于正常组和雄性大鼠。结论通过高脂饮食喂养成功建立了HFJ大鼠脂肪肝胰岛素抵抗疾病模型,与Wistar大鼠比较,HFJ大鼠特有的高血脂特征和近交系特点为进一步研究胰岛素抵抗脂肪肝的发病机制,筛选有效防治药物提供了良好的实验工具。更多还原

【Abstract】 Objective To create a insulin resistance fatty liver model by the HFJ inbred rats.Methods HFJ rats and Wistar rats,in half respectively male and female,which were randomly divided into a model and a control group.The model rats were fed a high-fat diet(44.2%of energy from fat) for 12 weeks,and the control group a standard diet(19.2%of energy from fat).To be weighed every week.Detected the fasting blood glucose,alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),total cholesterol(TC),HDLC,LDLC and fast insulin by drawing blood from Left ventricular.To measure liver wet weight by freeing liver,and calculated liver index.Rat liver was fixed with 10%formalin and embedded with paraffin.To assessment of hepatic steatosis and inflammation in all rats were observed under light microscope by HE stain.Results Liver tissue pathology showed that the hepatic cells of model group of HFJ and Wistar rats presented diffused macrovesicular steatosis,and control group rats showed no abnormal.All model group rats had elevated levels of AST,ALT,liver index and HOMA-IR,and there were no significant differences between HFJ rats and Wistar rats. Body weigh of the male model group of HFJ rats were higher than that of the control group(p<0.01),and there were no significant differences between model group and control group of Wistar rats(p>0.05).Compared with Wistar male rats, serum TG and TC of the HFJ male rats increased obviously.Serum TC of the female model group of HFJ and Wistar rats were higher than that of the control group and male rats.Conclusion A fatty liver and insulin resistance model was successfully developed in HFJ inbred rats fed a high-fat diet,which provided a useful experimental tool for elucidating pathogenesis and treatment of fatty liver.更多还原