【作者】 乔明琦； 张惠云； 魏盛； 孙鹏； 薛玲；

【Author】 Qiao Mingqi~*,Zhang Huiyun,Wei Sheng,Sun Peng,Xue Ling (Shandong University of Traditional Chinese Medicine,Key Laboratory of Traditional Chinese Medicine Classics Theory,Jinan 250355,China)

【机构】 山东中医药大学中医药经典理论教育部重点实验室；

【摘要】 目的本研究构建了经前期综合征(PMS)肝气逆证猕猴与正常猕猴大脑边缘系统正向与反向差减文库,并对猕猴cDNA文库进行了系统测序和分析,以期筛选出与.PMS肝气逆证相关的基因,尤其是与PMS肝气逆证有关的一些神经递质、性激素受体的基因。差减文库构建成功,为弄清PMS肝气逆证差异表达基因奠定了基础。更多还原

【Abstract】 In the present study,we constructed limbic system subtractive cDNA library of normal macaque and PMS liver-qi invasion macaque model,and more we had carried out systemic sequencing and analyzing to macaque cDNA library. Our puepose of thus work was to find out some genes relative to PMS liver-qi invasion,especially some genes of neurotransmitter and hormone receptors ralative to PMS liver-qi invasion.Subtractive cDNA library construction successfully provided solid foundation to clarify differential expression genes of PMS liver-qi invasion.更多还原