【作者】 甘宁； 黄文明； 杨德琴；

【Author】 Gan ning;Huang Wenming;Yang Deqin;Affiliated Stomatology Hospital of Chongqing Medical University,Endodontics;

【机构】 重庆医科大学附属口腔医院牙体牙髓科；

【摘要】 目的:激光共聚焦显微镜下观察四种常用根管冲洗药物对对生物膜状态下粪肠球菌的影响,比较不同冲洗剂对粪肠球菌生物膜的抑菌效果。方法:用盖玻片制备粪肠球菌生物膜,共18个样本,随机选取4孔作为空白对照,2孔用戊二醛固定后使用扫描电镜观察其生物膜形成状态,12孔随机分为4组,分别加入5.25%次氯酸钠溶液;3%H₂O₂;1%氯已定;MTAD各200μL,每种冲洗液作用3孔,作用时间120s后使用LIVE/DEAD细胞活性检测试剂盒对生物膜进行染色,再激光扫描共聚焦显微镜观察四种冲洗剂对生物膜状态下粪肠球菌的影响。结果:经四种冲洗药物作用以后,实验组生物膜内层活菌比例相比空白对照组未见明显减少,但中外2层的活菌比例均较空白对照组明显减少,差异统计学意义（P<0.05）。其药物作用效果为5.25%Naclo>MTAD>3%H₂O₂>1%CHX。结论:四种根管冲洗药物对粪肠球菌均有一定的抑菌作用,但均不能完全杀灭生物膜内的粪肠球菌,尤其是对生物膜内层的细菌作用较小。更多还原

【Abstract】 Objective:To compare the Inhibitory effect of four root canal irrigations on Enterococcus faecalis biofilm under confocal laser scanning microscopy.Methods:Preparation of Enterococcus faecalis biofilm coverslip were 18 samples,randomly selected four holes as a blank control,two holes using a scanning electron microscope after glutaraldehyde-fixed biofilm formation of the state,12 holes were randomly divided into four groups,were added 5.25%sodium hypochlorite solution,3%H₂O₂,1%chlorhexidine,MTAD each200 μL respectively,after 120 s using LIVE/DEAD cell activity assay kit for biofilm staining,and then laser scanning confocal microscope was uused to observe the impact of four Irrigations on Enterococcus faecalis biofilm state.Results:After washing four irrigations,the proportion of viable cells in the experimental group compared to the inner layer of biofilm control group showed no decrease,but the ratio of two layers of viable cells abroad than in the control group was significantly reduced,the difference was statistically significant（P<0.05）.their drug effect was 5.25%Naclo>MTAD>3%H₂O₂>1%CHX.Conclusion:Four root canal irrigations against Enterococcus faecalis had some antibacterial effect,but could not completely kill E.faecalis within the biofilm,especially smaller effect on biofilm bacterial lining.更多还原