【作者】 吴家媛； 贾谦； 何文喜； 倪龙兴； 牛忠英；

【Author】 Wu Jiayuan 1,Jia Qian 1,He Wenxi 1,Ni Longxing 1,Niu Zhongying2(1.Department of Endodontics,School of Stomatology,Fourth Military Medical University;2 306th Hospital.)

【机构】 第四军医大学口腔医学院牙体科； 解放军第306医院；

【摘要】 目的:探讨碱性成纤维细胞因子(Basic fibroblast growth factor,bFGF)对根尖牙乳头干细胞(Stem cells from the apical papilla,SCAP)成脂分化能力的影响。方法:将SCAP以2×104个细胞接种至60mm培养皿,15%FBS的a-MEM培养至细胞达到80%融合后分别以含或不含bFGF(5 ng/mL)成脂诱导液诱导,培养3w后,提取细胞总RNA,实时定量PCR检测成脂细胞标志基因PPAR-γ2(peroxisome proliferator-activatedreceptor-y2)基因表达情况,同时油红O染色检测细胞成脂能力。结果:油红O染色实验组(bFGF组)和对照组脂滴形成数量无差异;诱导1W时两组PPAR-γ2基因表达无差异,3w时,实验组PPAR-γ2基因表达高于对照组(p<0.05)。结论:bFGF可提高SCAP成脂潜能。更多还原

【Abstract】 Objective:To determine whether bFGF enhance the adipogenic differentiation potential of SCAP.Methods:Cells were seeded at 2×104 per well and maintained in standard medium containing 15%fetalbovine serum and antibiotics in 60mm dishes. After reached 80%confluence,the cells were exposed to adipogenic medium with or without bFGF for 3 weeks.Adipogenesis was stained with oil-red O.The expressions of adipocyte-specific gene expression(PPAR-y2)were detected by RT-PCR.Results: FGF(-)groups and FGF(+)groups showed similar oil-red O staining.The expression of PPAR-y2 at 1 week showed no different between two groups.The level of expression of PPAR-y2 in treated groups was higher than untreated bFGF groups at 3 weeks. Conclution:bFGF enhance the adipogenic differentiation potential of SCAP.更多还原