【作者】 王志刚； 施俊； 刘兴容；

【Author】 WANG Zhi-gang,SHI Jun,LIU Xing-rong.(Dept.of Oral Medicine,Hospital of Stomatology,Luzhou Medical College,Luzhou 646000,China)

【机构】 山东省淄博市中心医院口腔科； 湖北医药学院附属太和医院口腔教研室； 泸州医学院附属口腔医院口腔内科；

【摘要】 目的:分析蔗糖对不同龋敏感儿童口腔变异链球菌临床分离株不同基因型菌株的粘附能力的影响。方法:从不同龋敏感的3～5岁学龄前儿童口腔变异链球菌中选取56株变异链球菌临床分离株,采用Hamada的分光光度比浊法分析不同浓度蔗糖对变异链球菌临床分离株不同基因型菌株的粘附能力的影响。结果:在无蔗糖存在的情况下,不同龋敏感儿童口腔中变异链球菌临床分离株粘附比存在差异,高龋组>中龋组>无龋组,且差异具有统计学意义(p<0.05)。同一龋敏感儿童口腔中不同基因型变异链球菌临床分离株粘附比也存在差异,基因型越多,粘附比越大,统计分析差异具有显著性(p<0.05)。在不同蔗糖浓度下,不同龋敏感儿童口腔中变异链球菌临床分离株粘附比存在差异,4%组>1%组>0.5%组,即粘附比随蔗糖浓度升高而增高,差异具有统计学意义(p<0.05)。结论:不同龋敏感儿童口腔变异链球菌不同基因型菌株粘附能力存在差异,龋敏感性越高,基因型越多,粘附能力越强。蔗糖对其粘附能力具有明显的影响,蔗糖浓度越高,粘附能力越强,且明显大于无蔗糖组。更多还原

【Abstract】 OBJECTIVE:The aim of this study is to compare the effect of different sucrose concentration on adhesive ability of different genotype Streptococcus mutans isolated from children with different caries experience.METHODS:56 strains of S.mutans isolated from dental plaques of children aged from 3 to 5 with different caries experience were chosen as test bacteria.The adhesive ability of the strains were measured by UV spectrophotometer.RESULTS:There were significant difference in adhesive ability among the different genotypes of S.mutans isolated from children with different caries susceptibility(p < 0.05 ).The adhesive ability of the strains isolated from high caries- susceptibility is the highest,and that of caries-free group is the lowest.In the same group, The more genotypes the strain has,the more adhesive ability the strain produced,there were significant difference in the adhesion ability among the different genotypes of strains (p < 0.05 ).Sucrose plays an important role(?) the process of dental caries occurrence. With the concentration of sucrose the culture contains enhanced,the adhesive ability of the strain increased.CONCLUSION:There were significant difference in adhesive ability among the different genotypes of strains isolated from children with different caries experience.The higher caries experience,the higher adhesive ability the strain produced. The more genotypes the strain has,the more adhesive ability it had.Sucrose plays an important role in the process of dental caries occurrence.The higher concentration of sucrose the culture contains,the higher adhesion ability the strain has.更多还原