【作者】 郭婷； 曹罡； 余擎； 肖明振； 赵守亮； 吉兰；

【Author】 GUO Ting~1,CAO Gang~1,YU Qing~2,XIAO Ming-zhen~2,ZHAO Shou-liang~2,JI Lan~2.(1.Dental Department of Nanjing Military General Hospital.Nanjing 210002;2.Department of Operative Dentistry and Endodontics,Qindu Stomatological College,The Fourth Military Medical University.Xi’an 710032,Shanxi province,china)

【机构】 南京军区总医院口腔科； 第四军医大学口腔医学院；

【摘要】 目的:观察核心结合因子α1(core binding factorα1,cbfα1)对牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)基因启动活性的影响。方法:选择小鼠成牙本质细胞样细胞MDPC-23为实验细胞;DSPP基因上游2.6kb片段(-2475bp～+53bp)为启动子。通过采用瞬时转染、报告基因等方法,用SPSS10.0 for Windows软件对结果进行统计学分析,观察在MDPC-23中,cbfα1对DSPP基因启动子启动活性的影响。结果:在MDPC-23细胞中,pGL3-Enhancer-2.6K+pcDNA3-cbfα1共转染组荧光素酶的表达量明显小于pGL3-Enhancer-2.6K+pcDNA3共转染组(P<0.01)。结论:cbfα1对DSPP基因上游-2475bp～+53bp区域的启动活性有抑制作用。更多还原

【Abstract】 PURPOSE:To study the effect of core binding factorα1(cbfα1) on transcriptional regulation of mouse dentin sialophosphoprotein(DSPP) gene.METHODS:The MDPC-23 cells and the segment of nt -2475bp to +53bp were chosen.After co-transfected,the MDPC-23 cells were measured for luciferase activity using the dual luciferase reporter assay system.The results were analysised by softwere of SPSS10.0 for Windows.RESULTS:In MDPC-23 cells,the luciferase activity was significant low in the group of co-transfected with pGL3-Enhancer-2.6K and pcDNA3-cbfal than the group of pGL3-Enhancer-2.6K and pcDNA3 (P<0.01).CONCLUSION:Cbfal can reduce the activity of DSPP promoter include the segment of nt -2475bp to +53bp in MDPC-23 cells.This suggests that cbfal can transcriptionally regulate the expression of DSPP.更多还原