【作者】 刘建国； 季伯； 王凯； 李绍岳； 管晓燕； 王光平； 孙莉；

【Author】 Liu Jian-guo,Ji bo,Wang kai,Li Shao-yue,Guan Xiaoyan,Wang guang-pin,Sun Li.(The school of stomatology,Zunyi medical college, Zunyi 563003)

【机构】 遵义医学院口腔医学院口腔内科学教研室；

【摘要】 目的:利用氟病区煤烘玉米建立燃煤型氟斑牙大鼠模型,为进一步研究氟斑牙发生的分子机制提供实验基础。方法:将50只SD大鼠随机分为5组:高氟组、中氟组、低氟组、阳性对照组、阴性对照组,每组10只,雌雄各半。各实验组喂饲含不同比例的燃煤型氟病区煤烘玉米的饲料。实验开始和45天、90天时测量大鼠体重,记录氟斑牙发生情况、测量实验开始1月末和3月末牙齿长度、血氟、尿氟变化,采用HE染色的方法观察成釉细胞形态发生的改变。结果:①各实验组和阳性对照组均观察到氟斑牙,阴性对照组正常。②阴性对照组大鼠体重增加较实验组明显,差异有显著性(P<0.05)。③实验组大鼠血氟、尿氟同期水平依次为高氟组>中氟组>低氟组>阴性对照组,两两比较,差异有显著性(P<0.01)。④氟斑牙观察和记分:随着染氟时间的延长和染氟剂量的增加,实验组大鼠牙齿大部分棕色横纹消失,整个牙面呈白粉笔样,大多伴有牙体缺损;高氟组、中氟组与低氟组和阴性对照组比较,差异有显著性(P<0.01)。⑤牙齿长度测量:实验组大鼠牙齿长度在三月末明显短于一月末,实验组与阴性对照组比较有显著性差异(P<0.01)。⑥HE染色结果:实验组成釉细胞排列不规则,原有的高柱状结构变矮,细胞扭曲,釉质变薄。结论:①成功建立了燃煤型氟斑牙大鼠模型。②随着染氟时间的延长和染氟剂量的增加,大鼠氟斑牙的严重程度也随着增加。③过量的氟可导致大鼠成釉细胞由原有的高柱状向矮立方转化,发生排列紊乱、扭曲等形态改变。更多还原

【Abstract】 Objective:To establish rat model of dental fluorosis with corns dried by burning coal.To prove the established animal model of dental fluorosis was successful by index detection during experimental process.Methods:Fifty SD rats were randomly divided into 5 groups(the number of male and female in each group was the same respectively):the high-dose fluoride group,the middle-dose fluoride group,the low-dose fluoride group,the positive control group and the negative control group.To measure rats’ weight once a week,all rats were killed by means of decapitation,then were examined dental fluorosis,tooth length,fluoride in urine and serum and were observed the change of ameloblast in morphology by HE staining.Result:There were fluorosis in each experimental group and it is all right for rats in the negative control group.①Significant differences of rats weight in each group were recorded between experimental groups and negative control group(P<0.05).②Compared with negative control group,fluoride level in urine and serum was higher in experimental groups,which was statistically significant(P<0.001).③Dental fluorosis scores:There were dental fluorosis in each experimental group and it is all right for rats in the negative control group.The tooth color change from brown to amber,white opaque tissue appear in partial area(P<0.001).④Measurement of tooth length:Significant differences of tooth length in each experimental group were recorded before and after experiment,tooth length of experimental groups was shorter than those of control group(P<0.001).⑤HE staining showed that ameloblast of experimental groups from high column form change into multilayer,majority of ameloblasts distorted and enamel become thinly.Conclusion:①The animal model of dental fluorosis established successful after three months.②Along with increasing fluoride intake and prolonged fluoride intake time,serious degrees and incidence rates of dental fluorosis increase striking.③Excessive fluoride level induce the change of ameloblast in morphology, arrange disorderly and distorted.更多还原