【作者】 张翀； 赵宝红； 赵震锦； 邓春富；

【Author】 ZHANG Chong~1,ZHAO Bao-hong~1,ZHAO Zhen-jin~2,DENG Chun-fu~(1(?)) (1.Department of Implant Center,2.Department of Orthodontics,School of Stomatology, China Medical University,Shenyang 110002,Liaoning Province,China)

【机构】 中国医科大学口腔医学院种植中心； 中国医科大学口腔医学院正畸科；

【摘要】 目的:评价微弧氧化(Micro-arc Oxidation,MAO)与离子束辅助沉积(Ion Beam Assisted Deposition,IBAD)相结合的处理方法对钛种植材料表面微形态及成骨活性的影响。方法:利用IBAD技术,在MAO钛材料表面制备羟基磷灰石(Hydroxyapatite,HA)涂层,将钛种植材料按照不同的表面处理方法分为机械抛光组(SO)、MAO组(Sl)和MAO与IBAD相结合组(S2),以S0作为对照组。利用表面形貌仪分析3组材料表面形貌及粗糙度,利用激光共聚焦显微镜观察成骨细胞在材料表面黏附铺展状态;采用MTT法和碱性磷酸酶(ALP)活性测定的方法,检测不同处理方法对材料成骨活性的影响。所得数据利用SPSS11.0软件包进行单因素方差分析。结果:S2组材料表面粗糙度略小于S1组;复合培养5d后,S2组细胞增殖水平明显高于其他2组;10d后,2粗糙组ALP活性明显高于S0组。结论:MAO与IBAD技术相结合,在MAO表面制备HA活性层,能够明显的促进成骨细胞的黏附增殖,促进成骨细胞的矿化。更多还原

【Abstract】 Purpose:To evaluate the bioactivity of titanium surface treated by micro-arc oxidation(MAO)combined with ion beam assisted deposition(IBAD).Methods:Titanium surfaces were treated by machined(SO),micro-arc oxidation(S1)and micro -arc oxidation combined with 1BAD(S2)respectively.The topography and roughness of surfaces were determined by profilometer, and the bioactivity of the surfaces were evaluated by CLSM,MTF assay and ALP activity of the cells.Data were analyzed by one-way ANOVA,SPSS11.0.Results:The surfaces of S2 were smoother than those of S1.On day 5,the OD value of S2 group was the highest among the three groups.10 days later,ALP activity on S1 and S2 groups were higher than that of SO.Conclusions:It was concluded that IBAD technique which provides HA coating on MAO surface could reinforce the bioactivity of micro-arc oxidation and could have a rapid osseointegration.Supported by Shenyang science and technology issue(1081234-1-00).更多还原