【作者】 张铁； 王春光； 韩伟； 李清艳； 吕建存；

【Author】 Zhang Tie~1 Wang Chunguang~1 Han Wei~2 Li Qingyan~1 Lui Jiancun~1, (1.College of Traditional Chinese veterinarymedicine,Agricultural University of Hebei,Dingzhou,Hebei 073000) (2.College of Veterinary Medicine;China Agricultural University;Beijing 100094)

【机构】 河北农业大学中兽医学院； 中国农业大学动物医学院；

【摘要】 对分离自保定(BD)、秦皇岛(QHD)和北京(BJ)三个地区规模化养鸡场的病死鸡体内的38株大肠杆菌,采用超声波裂解和N-十二烷基肌胺酸钠进行Omp的提取,利用SDS-PAGE进行Omp分型。结果发现:共有3个Omp型,其中Omp-1型为078、088、02、018、093、0765个血清型分离株所共有,Omp-Ⅱ型多为076血清型分离株,Omp-Ⅲ型为093、078、088、02分离株所共有。表明同一血清型的菌株可能属于完全不同的Omp型,而血清学上毫不相关的分离株之间却可具有相同的Omp型,证实了同一血清型的分离株之间可发生遗传分化,而不同血清型的分离株之间也可具有不同程度的遗传相关性。更多还原

【Abstract】 The outer membrane protein（OMP） patterns were extracted from 38 strains avian Escherichia coli which were isolated from chicken breeding farms in Baoding,Qin Huangdao and Beijing areas by using supersonic lysis and Sarkosyl.OMP were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis （SDS-PAGE）.The results indicated three different Omp patterns are identified with these 38 strains,Of which,O₇₈,O₈₈,O₂,O₁₈,O₉₃,O₇₆ appear Omp-1 pattern,O₇₆ appear Omp-Ⅱpattern, O₉₃,O₇₈,O₈₈,O₂ appear Omp-Ⅲpattern.And different serotype strains have the same Omp patterns, the same serotype strains may have different Omp patterns.The means of O serotype patterns could not reflect genetic relativity among strains completely.更多还原