【作者】 利凯； 马利芹； 徐彤； 霍亚男； 吴占福；

【Author】 Huo Ya-nan LI Kai MA Li-qin XU Tong HUO Ya-nan WU Zan-fu (College of Animal Science and Technology,Hebei North University,Zhangjiakou 075131,China)

【机构】 河北北方学院动物科技学院；

【摘要】 目的探讨重组溶葡萄球菌酶对小鼠实验性乳腺炎的治疗效果方法利用微量肉汤稀释法测定重组溶葡萄球菌酶对乳源金黄色葡萄球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC)。15只泌乳8～10天的BALC/C母鼠随机分成3组:正常组、阳性对照组和试验组。阳性对照组和试验组母鼠经第4、5对乳腺乳头管注入50μl 2×10~5CFU细菌悬液。试验组攻菌出现症状后,每个乳腺经乳头管注入50μl IU/ml的重组溶葡萄球菌酶稀释液,间隔12h一次。观察小鼠的临床症状,测定体温、血常规等指标。接菌66h后,颈静脉放血处死小鼠,取小鼠乳腺进行细菌计数和病理组织切片制作。结果实验测得重组溶葡萄球菌酶对金黄色葡萄球菌的MIC值为0.001U/ml,MBC值为0.063U/ml。病理组织观察显示,正常组腺泡结构完整,阳性对照组乳腺组织局部破坏,间质增宽,炎性细胞浸润严重,试验组组织结构基本正常,组织轻度充血。结论结果表明重组溶葡萄球菌酶能有效减少细菌感染,降低组织损伤,对乳腺有一定的保护作用。更多还原

【Abstract】 Objective In order to study the treatment result of recombinant lysostaphin on experimental mastitis in mice induced by staphylococcus aureus.Methods A trace broth dilution was used for determination of the MICs ang MBCs of recombinant lysostaphin against staphylococcus aureus clinically isolated from mastitis cows.Fifteen pregnant BALB/C mice randomly divided into normal control group,positive control group and treatment group.Five mice in each group.50μl 2.0×10CFU Staphylococcus aureus was inoculated into the fourth mammary gland on 8-10d after parturition.50μl 1U/ml recombinant lysostaphin was injected into the cavity of every mammary gland after symptoms of treatment group was obvious,and once every 12 hours.The clinical symptoms were observed.The body temperature,blood and other indicators were measured.66h after inoculation,the mice were euthanatized and the mammary glands were removed and fixed for histopathologic evaluations.Results The MICs of recombinant lysostaphin was 0.001U/ml and the MBCs was 0.063U/ml.Histopathologic examination of mammary gland revealed that the mammary glands frame of normal group was integrated.Some mammary gland frame of positive control group was damaged, the interstitial width increased,and a large number of inflammatory cells aggregated.The frame of treatment group was basically normal,organization was lightly congestive.Conclusion Recombinant Lysostaphin was effective in reducing the bacterial infection and tissue injury.The mammary glands can be protected by recombinant lysostaphin.更多还原