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用RT-PCR检测Ⅰ型鸭肝炎病毒
Diagnosis of Duck Hepatitis Virus typeⅠby RT- PCR
【作者】 周珍辉; 王弋嘉; 向双云; 田锦; 田璐; 陈万荣; 周育森;
【Author】 ZHOU Zhen-hui WANG Yi-jia XIANG Shuang-yun TIAN Jin TIAN Lu CHEN Wan-rong ZHOU Yu-sen (1.Department of Animal Husbandry & Veterinary,Beijing Agricultural Vocational College,Fangshan 102442) (2.Beijing Institute of Microbiology and Epidemiology,100071)
【机构】 北京农业职业学院畜牧兽医系; 北京微生物流行病研究所;
【摘要】 取北京郊区某鸭场的病鸭肝脏制备病料,接种12日龄鸭胚尿囊腔及2日龄健康雏鸭,应用RT-PCR方法对病鸭的肝脏悬液及鸭胚尿囊液进行检测并测序,确定所分离的病毒为Ⅰ型鸭肝炎病毒。
【Abstract】 Livers of diseased ducks in Beijing district were made disease materials.Disease materials were inoculated in 12 days duck embryo allantoid cavity and healthy ducks of 2 days.Livers of diseased ducks and duck embryo allantoid cavity liquid were proved by RT-PCR and sequence mensuration that the serotype of the isolated virus was the TypeⅠ.
- 【会议录名称】 京津冀畜牧兽医科技创新交流会暨新思想、新观点、新方法论坛论文集
- 【会议名称】京津冀畜牧兽医科技创新交流会暨新思想、新观点、新方法论坛
- 【会议时间】2008-12
- 【会议地点】中国河北保定
- 【分类号】S858.32
- 【主办单位】北京市畜牧兽医学会、天津市畜牧兽医学会、河北省畜牧兽医学会