【作者】 王金秋； 林德贵； 王雷； 宋筱瑜； 韩春杨；

【Author】 WANG Jin-qiu~1 LIN De-gui~(2*) WANG Lei~3 SONG Xiao-yu~2 HAN Chun -yang~2 (1.Beijing Vocational College of Agriculture,Beijing 102442) (2.College of Veterinary Medicine,China Agricultural University,Beijing 100094) (3.University of Minnesota Cancer Center,Minneapolis 55455)

【机构】 北京农业职业学院； 中国农业大学动物医学院； 美国明尼苏达癌症研究中心；

【摘要】 为研究含硒化合物对小鼠氧化性损伤的保护作用,将60只ICR小鼠随机分为6组:对照组、辐射组、高剂量和低剂量亚硒酸钠（Na₂SeO₃）组,高剂量和低剂量甲基硒代半胱氨酸（MSeC）组,每组10只。每天灌胃给药0.3mL,前2组灌服同等体积的蒸馏水,2个Na₂SeO₃组分别按Se含量20、10μg/mL灌胃给予Na₂SeO₃,2个MSeC组分别按Se含量20、10μg/mL灌胃给予MSeC,连续14d。灌胃7d后,除对照组外,其他组小鼠进行一次总剂量为4.5Gy的全身辐照,建立小鼠氧化性损伤模型。测定有关免疫功能和抗氧化能力指标。结果:辐照后第3 d接受辐射各组血液中白细胞数量明显下降;辐照组和灌服高、低剂量的Na₂SeO₃组在辐照后第6天血液中白细胞数量继续下降,而灌服MSeC的2组稳定上升;辐射组GSH-Px、SOD活性、GSH水平下降,MDA水平升高,补硒各组的各项指标与辐照组差异显著或极显著,与对照组无显著差异;补硒各组的脾脏指数（SI）和胸腺指数（TI）均辐射组有显著差异。可见辐射会导致小鼠抗氧化能力下降,含硒化合物,尤其是有机硒化合物甲基硒代半胱氨酸（MSeC）有抗氧化损伤的作用,且安全剂量范围内的高剂量组的保护作用更明显。更多还原

【Abstract】 To study effects of Selenium Compounds on the oxidative damage,sixty ICR mice were randomly divided into six groups:control group,radiation group,high and low dose Na₂SeO₃ groups,high and low dose MSeC groups,10 mice per group.Intragastric administration of 0.3ml Solutions once per day and lasted for 14 d,high and low dose Na₂SeO₃ groups were given Na₂SeO₃（20μg/mL and 10μg/mL）,high and low dose of MSeC groups were given MSeC（20μg/mL and 10μg/mL）,same volume water was given to control group and radiation group instead.7 days later all groups accepted one time radiation except the control group,at the dosage of 4.5Gy,and the oxidative damage model was established.During the experiment, immunity and antioxidation functions were measured respectively.3 days after radiation,WBC of groups accepted radiation decreased significantly;6 days after radiation,WBC of radiation group and the two Na₂SeO₃ groups continue declined,and the two MSeC groups increased steadily.In radiation group the GSH -Px,SOD activities,GSH decreased significantly and MDA increased significantly compared with normal control group.There was no difference between normal control group and the groups accepting selenium compounds.The Spleen Index（SI） and Thymus Index（TI） of those accepting selenium compounds groups had significant difference with radiation group.Radiation results in oxidative damage and selenium compounds, especially methylselenocysteine（MSeC）has protect effects on anti-oxidative damage,and the protect effect of high dosage within the range of safe dosage is obvious.更多还原