【作者】 杨勇； 庹德财； 沈文涛； 言普； 黎小瑛； 周鹏；

【Author】 Yang Yong~(1,2),Tuo Decai~(1,2),Shen Wentao~2,Yah Pu~2,Li Xiaoying~2,Zhou Peng~(1,2*) 1 Agriculture College,Hainan University,Haikou,Hainan 570228,China 2 Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou,Hainan 571101,China

【机构】 中国热带农业科学院热带生物技术研究所；

【摘要】 利用马铃薯Y病毒属的简并引物对3个海南感病番木瓜植株的叶片样品进行RT-PCR检测,皆获得了预期大小约1700bp的特异性条带,将其克隆到T载体上进行测序和BLAST分析,结果显示:所得序列与GenBank中收录的番木瓜畸形花叶病毒(Papaya leaf distottion mosaic virus,PLDMV)基因序列相似性达到88%～96%,序列包含部分NIb蛋白基因、外壳蛋白(cP)基因和3’端非编码区。这是首次在我国海南地区报道发现番木瓜感染了PLDMV。但是基于PLDMV外壳蛋白基因的系统进化分析结果还不足以证明在海南发现的PLDMV与其他地区的PLDMV为同一进化簇。更多还原

【Abstract】 the diseased papaya displaying mosaic and ringspot symptom leaves in Hainan Island was detected using RT-PCR~ based on potyvirus specific primers.A product about 1700bp was generated and then cloned into pMD-18-T vector. Sequencing and BLAST results showed that the nucleotide sequence share the identities of 88%to 96%with those of other strains of papaya leaf distortion mosaic virus(PLDMV) obtained from GenBank.The sequence contained part of Nib gene, coat protein(CP) gene and 3’ non-coding region.So the diseased papaya was identified to be infected by PLDMV.This was the first report of PLDMV infecting papaya in Hainan.The phylogenetic analysis based on the CP gene was not enough to identify that they belong to same cluster.更多还原