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营养因素与培养条件对Pilidium concavum病菌菌丝生长及产孢量的影响
Effects of nutritional factors and culture conditions on the growth and sporulation of Pilidium concavum
【Author】 Duan Yabing,Yu Zhenzhen,Chen Yanli,Kang Yebin (College of Forestry,Henan University of Science and Technology,Luoyang 471003,China)
【机构】 河南科技大学林学院；
【摘要】 为了探讨不同培养基、碳氮源、温度、pH值、光照对Pilidium concavum(Desm.)Hohn.菌丝生长及分生孢子产量的影响。试验采用十字交叉法测量菌丝生长量,用血球计数板法测量产孢量。结果表明,该菌在PDA上生长最适,在PYA上产孢最多。在最适培养基上培养,菌丝生长的适宜温度为15～30℃,最适温度为25℃,产孢最适温度为30℃;在pH值6～11范围内该菌均能生长和产孢,菌丝生长的最适pH值为6,pH值为11产孢最多;光照对菌丝生长、产孢无显著影响;在供试的碳氮源中,菌丝生长及产孢以葡萄糖、酵母膏最适。
【Abstract】 In order to explore the effects of culture medium,carbon,nitrogen,temperature,pH and light on the growth and sporulation of Pilidium concavum(Desm.) Hohn..Measured the mycelial growth with criss-cross and the sporulation by blood counting chamber.The results showed that the optimum grown was PDA,the optimum sporulation was PYA.The optimum grown temperature was 25℃,the optimum temperature for sporulation was 30℃.The optimum growth pH was 6,sporulation was a maximum of pH 11.Light had no significant effect on mycelial growth and sporulation.Glucose and yeast were the most suitable carbon and nitrogen sources for the growth of mycelia and sporulation.
【Key words】 Pilidium concavum； hypha； conidiophore； nutritional factors； culture conditions；
- 【会议录名称】 河南省植保学会第九次、河南省昆虫学会第八次、河南省植病学会第三次会员代表大会暨学术讨论会论文集
- 【会议名称】河南省植保学会第九次、河南省昆虫学会第八次、河南省植病学会第三次会员代表大会暨学术讨论会
- 【会议时间】2009-11-19
- 【会议地点】中国河南南阳
- 【分类号】S436.8
- 【主办单位】河南省植物保护学会、河南省昆虫学会、河南省植物病理学会