【作者】 段亚冰； 余真真； 陈艳利； 康业斌；

【Author】 Duan Yabing,Yu Zhenzhen,Chen Yanli,Kang Yebin (College of Forestry,Henan University of Science and Technology,Luoyang 471003,China)

【机构】 河南科技大学林学院；

【摘要】 为了探讨不同培养基、碳氮源、温度、pH值、光照对Pilidium concavum(Desm.)Hohn.菌丝生长及分生孢子产量的影响。试验采用十字交叉法测量菌丝生长量,用血球计数板法测量产孢量。结果表明,该菌在PDA上生长最适,在PYA上产孢最多。在最适培养基上培养,菌丝生长的适宜温度为15～30℃,最适温度为25℃,产孢最适温度为30℃;在pH值6～11范围内该菌均能生长和产孢,菌丝生长的最适pH值为6,pH值为11产孢最多;光照对菌丝生长、产孢无显著影响;在供试的碳氮源中,菌丝生长及产孢以葡萄糖、酵母膏最适。更多还原

【Abstract】 In order to explore the effects of culture medium,carbon,nitrogen,temperature,pH and light on the growth and sporulation of Pilidium concavum(Desm.) Hohn..Measured the mycelial growth with criss-cross and the sporulation by blood counting chamber.The results showed that the optimum grown was PDA,the optimum sporulation was PYA.The optimum grown temperature was 25℃,the optimum temperature for sporulation was 30℃.The optimum growth pH was 6,sporulation was a maximum of pH 11.Light had no significant effect on mycelial growth and sporulation.Glucose and yeast were the most suitable carbon and nitrogen sources for the growth of mycelia and sporulation.更多还原