【作者】 蒋武生； 张晓伟； 原玉香； 姚秋菊； 耿建峰； 张强； 王志勇；

【Author】 JIANG Wu-sheng,ZHANG Xiao-wei,YUAN Yu-xiang,YAO Qiu-ju, GENG Jian-feng,ZHANG Qiang,WANG Zhi-yong (Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002)

【机构】 河南省农业科学院园艺研究所；

【摘要】 13个甘蓝品种（品系）在NLN-13液体培养基中有10个品种诱导出胚。每花蕾产胚在0～1.8之间。比较NLN培养基中添加激素和活性炭的处理结果表明,NLN+13%蔗糖+活性炭0.5mg/L效果较好,胚诱导率提高3倍。接种后小孢子在33℃高温预处理24h产胚量较高。B₅+6-BA0.2mg/L+3%蔗糖+1%琼脂培养基有利于小孢子胚长成植株。更多还原

【Abstract】 Isolated microspores from 13 varieties of rape were cultured in NLN-13 Liquid medium, 10varieties had produced microspore-derived embryoids.Embryo yield per bud was from 0 to 1.8. Hormone concentrations and active charcoal in NLN medium had been compared.NLN with 13% sucrose and active charcoal 0.5mg/L was better in production of microspore-derived embryoids. Average embryo yield was higher in 33℃high temperature treatment from 24 hour. Microspore-derived embryoids on B₅ medium with 6-BA0.2mg/L and 3%Sucrose and 1%agar was good to regenerated plants.更多还原