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大马士革玫瑰RdGASA4-like基因启动子的分离及其在甘蓝中的瞬时表达分析
Isolation and Transient Expression Analysis in Cabbage of the Promoter of RdGASA4-like from Damask rose
【作者】 张强; 张晓伟; 原玉香; 蒋武生; 姚秋菊; 赵艳艳;
【Author】 Zhang Qiang~1,Zhang~1 Xiaowei~1,Yuan Yuxiang~1,Jiang Wusheng~1,Yao Qiuju~1,Zhao Yanyan~1 (Institute of Horticulture Science,Henan Academy of Agriculture Science,Zhengzhou 450002)
【机构】 河南省农业科学院园艺研究所;
【摘要】 在前期的研究中,一个玫瑰RdGASA4-like基因的cDNA和DNA序列全长利用同源克隆结合RACE的方法首次得到克隆。为深入了解该基因在赤霉素调控植物发育过程中的作用,通过TAIL-PCR的方法首次克隆该基因起始密码子ATG上游685bp的5’-UTR和部分启动子序列,在启动子序列分析的基础上,构建其与GUS基因融合表达载体,命名为pBI-GP685。通过农杆菌介导的方法对甘蓝外植体子叶-子叶柄进行启动子瞬时表达研究,结果表明分离得到的启动子具有启动报告基因GUS表达的活性。
【Abstract】 In previous study,the full length DNA and cDNA of the RdGASA4-like gene had been isolated from Damask rose(Rosa damascena) through homology cloning combined with rapid amplification of cDNA ends(RACE).In order to further understand the role of RdGASA4-like in the regulating network on plant development of gibberellic acid,its 685bp 5’-UTR and partial sequence upstream ATG of RdGASA4-like was obtained by Thermal Asymmetric Interlaced Polymerase Chain Reaction(TAIL-PCR).Based on sequence analysis,it was fused to a uid A gene to construct plant expression vector,named as pBI-GP685.By agrobacterium-mediated method, the explants of petiole with cotyledon of Cabbage were transformed.The transient expression study showed that the obtained promoter of RdGASA4-like was active and can drive the uid A by GUS assay analysis.
【Key words】 RdGASA4-like; isolation; promoter; transient expression;
- 【会议录名称】 河南省植物生理学会三十周年庆典暨学术研讨会论文集
- 【会议名称】河南省植物生理学会三十周年庆典暨学术研讨会
- 【会议时间】2010-12-18
- 【会议地点】中国河南郑州
- 【分类号】S685.12
- 【主办单位】河南省植物生理学会(Henan Society for Plant Physiology)