【作者】 张强； 张晓伟； 原玉香； 蒋武生； 姚秋菊； 赵艳艳；

【Author】 Zhang Qiang~1,Zhang~1 Xiaowei~1,Yuan Yuxiang~1,Jiang Wusheng~1,Yao Qiuju~1,Zhao Yanyan~1 (Institute of Horticulture Science,Henan Academy of Agriculture Science,Zhengzhou 450002)

【机构】 河南省农业科学院园艺研究所；

【摘要】 在前期的研究中,一个玫瑰RdGASA4-like基因的cDNA和DNA序列全长利用同源克隆结合RACE的方法首次得到克隆。为深入了解该基因在赤霉素调控植物发育过程中的作用,通过TAIL-PCR的方法首次克隆该基因起始密码子ATG上游685bp的5’-UTR和部分启动子序列,在启动子序列分析的基础上,构建其与GUS基因融合表达载体,命名为pBI-GP685。通过农杆菌介导的方法对甘蓝外植体子叶-子叶柄进行启动子瞬时表达研究,结果表明分离得到的启动子具有启动报告基因GUS表达的活性。更多还原

【Abstract】 In previous study,the full length DNA and cDNA of the RdGASA4-like gene had been isolated from Damask rose(Rosa damascena) through homology cloning combined with rapid amplification of cDNA ends(RACE).In order to further understand the role of RdGASA4-like in the regulating network on plant development of gibberellic acid,its 685bp 5’-UTR and partial sequence upstream ATG of RdGASA4-like was obtained by Thermal Asymmetric Interlaced Polymerase Chain Reaction(TAIL-PCR).Based on sequence analysis,it was fused to a uid A gene to construct plant expression vector,named as pBI-GP685.By agrobacterium-mediated method, the explants of petiole with cotyledon of Cabbage were transformed.The transient expression study showed that the obtained promoter of RdGASA4-like was active and can drive the uid A by GUS assay analysis.更多还原