【作者】 梁冰； 李淑芳； 王骁宇； 刘锦红； 沈祥春；

【Author】 LIANG Bing,LI Shu-fang,Wang Xiao-yu,Liu Jin-hong,Shen Xiang-chun (Department of Pharmacology,Guiyang Medical University,Guiyang 55004,China)

【机构】 贵阳医学院药理学教研室；

【摘要】 目的 :探讨氧化苦参碱对环磷酰胺（CTX）抗S₁₈₀小鼠的增效减毒作用。方法 :建立S₁₈₀荷瘤小鼠模型,增效实验设立:模型组、环磷酰胺10mg/kg、20mg/kg组、环磷酰胺（5mg/kg）+氧化苦参碱（50mg/kg）组、环磷酰胺（10mg/kg）+氧化苦参碱（50mg/kg）组,观察肿瘤抑制率,胸腺和脾脏指数。减毒实验设立:正常对照组、环磷酰胺100mg/kg组、环磷酰胺（100mg/kg）+氧化苦参碱低剂量（25mg/kg）组、环磷酰胺（100mg/kg）+氧化苦参碱中剂量（50mg/kg）组、环磷酰胺（100mg/kg）+氧化苦参碱高剂量（100mg/kg）组,观察白细胞数,胸腺和脾脏指数,检测肝、肾功能。结果 :增效实验中,各用药组与模型组相比均能明显提高肿瘤抑制率,环磷酰胺+氧化苦参碱组与单用环磷酰胺组相比胸腺指数升高。减毒实验中,环磷酰胺+氧化苦参碱高、低剂量组与单用环磷酰胺组相比白细胞数量增加;高、中剂量氧化苦参碱与环磷酰胺合用后天门冬氨酸含量降低;高剂量氧化苦参碱与环磷酰胺合用后丙氨酸氨基转移酶含量降低;中剂量氧化苦参和环磷酰胺合用后尿素含量增高。结论 :氧化苦参碱具有抑制肿瘤生长的作用,与环磷酰胺合用不仅能提高抑瘤率和白细胞数,还能减轻环磷酰胺引起的肝损害。更多还原

【Abstract】 Objective:To investigate synergism and attenuation effects of oxymatrine（OMT） on cyclophosphamide（CTX） anti-S₁₈₀ tumor-bearing mice.Methods:The model of S₁₈₀ tumor-bearing mice was established.Synergism experimental set up:the model group,cyclophosphamide 10mg/kg, 20mg/kg group,cyclophosphamide（5mg/kg） + oxymatrine（50mg/kg） group,cyclophosphamide （10mg/kg） + oxymatrine（50mg/kg） group.The tumor inhibition rate,thymus and spleen index were be observed..Attenuation experimental set up:control group,cyclophosphamide 100mg/kg group,cyclophosphamide（100mg/kg） + oxymatrine low-dose（25mg/kg） group,cyclophosphamide （100mg/kg） + oxidation middle-dose（50mg/kg） group,cyclophosphamide（100mg/kg） + oxymatrine high-dose（100mg/kg） group.White blood cell count,thymus and spleen index were be accounted.Liver and kidney function were be detected.Results:On the synergism experiments, compared with the model group,the treatment group could significantly improve tumor inhibition rate. Compared with cyclophosphamide group,cyclophosphamide combination with with oxymatrine group the index thymus was risen.Attenuation experiments,compared with cyclophosphamide group, oxymatrine high-and low-dose combination with cyclophosphamide group the number of white blood cells was increased.Cyclophosphamide combination with high-dose Oxymatrine aspartate content reduced.Cyclophosphamide combined with high-dose oxymatrine alanine aminotransferase levels reduced.Cyclophosphamide combined with middle-dose oxymatrine urea levels increased. Conclusion:Oxymatrine can inhibit tumor growth,combined with cyclophosphamide not only can improve the inhibition rate and white blood cell count,but also reduce the cyclophosphamide-induced liver damage.更多还原