【作者】 谢广云； 张杰； 肖杨； 韩莹； 刘娜； 孙金秀； 牟颖； 韩四海； 黄沛力； 孙志伟；

【Author】 XIE Guangyun,HAN Ying,LIU Na,SUN Zhiwei,HUANG Peili 1.School of Public and Familay Medince,Capital Medical University,Beijing 100069 2.Insititue for Occupational Health and Poisoning Control,Chinese Center for Disease Control and Prevention,Beijing 100050

【机构】 首都医科大学公共卫生及家庭医学学院； 中国疾病预防控制中心职业卫生与中毒控制所； 浙江大学工业控制技术国家重点实验室智能系统与控制研究所分析仪器研究中心；

【摘要】 目的探讨碲化镉量子点(CdTe QDs)对小鼠肝脏的氧化应激及其DNA损伤作用。方法将30只雄性ICR小鼠随机分成5组,即阴性对照组、0.375、37.5、375nmol/ml染毒组和阳性对照组,染毒24h后断头处死动物。用低温电子顺磁共振(EPR)检测小鼠肝脏组织自由基水平的变化;用单细胞凝胶电泳(SCGE)检测小鼠肝DNA损伤。结果染毒24h时后,随着CdTe QDs染毒剂量的增加,低、中、高染毒组的小鼠肝脏组织自由基水平与对照组比较有统计学意义的增高(P<0.05,P<0.01);小鼠肝细胞SCGE检测彗星尾长、Olive尾矩、尾DNA%、头尾比与对照组比较具有统计学意义的增高(P<0.01)。结论 CdTe QDs可导致小鼠肝细胞产生氧化应激和DNA损伤,并有一定的剂量-效应关系。更多还原

【Abstract】 Objective To study the effects of CdTe QDs on oxidative stress and DNA damage of liver cell in mice.Methods 30 ICR male mice were randomly divided into 5 groups,namely,groups of of negative control,0.375、37.5、375nmol/ml and positive control.Mice were administered with CdTe QDs by through intravenous injection for 24h,and then Through EPR and SCGE,free radical and DNA damage was detected resperctively.Results The EPR showed that compared with the control, along with the increase of the dosage,the oxygen free radical increased,and the there was a significant different among groups(P<0.05.P<0.01);The SCGE result of mice liver showed that tail length,Oliver tail moment,tail DNA(%) and ratio of tail/head increased with the the dosage increase, and significant difference wase found among all groups(P<0.01).Conclusion CdTe QDs could induce oxidative stress and DNA damage of liver cells of mice.更多还原