【作者】 孙立； 田晓滨； 杨述华； 胡如印； 汪雷； 陆延盛； 张宇坤； 傅德皓；

【Author】 SUN Li,TIAN Xiaobing,YANG Shuhua,et al.Department of orthopedics,People’s Hospital of Guizhou Province,Guiyang Guizhou,550002,P.R.China

【机构】 贵州省人民医院骨科； 华中科技大学同济医学院附属协和医院骨科；

【摘要】 目的:观察骨形成蛋白2（BMP2）、血管内皮生长因子₁₆₅(VEGF₁₆₅)双基因转染的小鼠骨髓基质千细胞是否具有异位诱导成骨能力。方法:脂质体介导下将双基因真核表达质粒pIRES-BMP2-VEGF₁₆₅导入小鼠骨髓基质干细胞。用RT-PCR和免疫组织化学方法观察BMP2和VEGF₁₆₅双基因在小鼠骨髓基质干细胞内的表达;将转染BMP2和VEGF₁₆₅双基因的小鼠骨髓基质干细胞植入4只裸鼠大腿后群肌袋内,4周后用影像学和组织学方法观察体内诱导成骨作用。结果:转染BMP2、VEGF₁₆₅的小鼠骨髓基质干细胞有明显的BMP2和VEGF₁₆₅ mRNA及其蛋白表达,此细胞植入裸鼠大腿后群肌袋,4周后有大量异位骨形成。结论:转染BMP2和VEGF₁₆₅双基因的小鼠骨髓基质干细胞具有较强诱导异位成骨能力。更多还原

【Abstract】 Objective To evaluate the osteogenic potential efficacy of mouse bone marrow stromal cells（mMSCs） transfected with bone morphogenetic protein 2（BMP2） and vascular endothelial growth factor₁₆₅(VEGF₁₆₅) genes. Methods The mMSCs were transfected with pIRES - BMP2 - VEGF₁₆₅ by liposome - mediated method.The co - expression of BMP2 and VEGF₁₆₅ genes were determined by RT - PCR and immunohistochemical analysis.The transfected cells were injected into the thigh muscle pouches of four nude mice.The osteoinductivity of the transduced cells were evaluated by radiography and histology analysis in 4 weeks after injection. Results RT- PCR and immunohistochemical analysis showed that these transfected cells indeed could produce the mRNA and proteins of the BMP2 and VEGF₁₆₅.The ectopic bones formed in the thigh muscle pouches of the nude mice were obviously observed 4 weeks after cell injection. Conclusion The mMSCs transfected with BMP2 and VEGF₁₆₅ genes can induce ectopic osteogenesis of mouse marrow stromal cells.更多还原