【作者】 叶丽卡； 谢志红； 陈瑞卿；

【Author】 YE Li-ka,XIE Zhi-hong,CHEN Rui-qing (The Second Affiliated Hospital of GuangZhou Medical College 510260,China)

【机构】 广州医学院第二附属医院；

【摘要】 目的:建立用高效液相色谱法测定人血浆中5-FU的浓度。方法:采用UltimateC₁₈色谱柱（250mm×4.5mm,5μm）;流动相:10mmol·L^-1KH₂PO₄缓冲溶液;流速:1.0mL·min^-1;检测波长:265nm;柱温:40℃。结果:5-FU在0.48μg·mL^-1～61.4μg·mL^-1范围内线性关系良好（r=0.998）,最低检测浓度0.48μg·m^-1,日内和日间RSD分别为5.12%～9.98%（n=5）和5.28%～10.95%（n=5）。室温放置,冷冻保存,反复冻融条件下稳定性良好。结论:该法具有操作简便可靠,准确,稳定性高等特点,适合应用于人血浆中5-FU的浓度测定。更多还原

【Abstract】 OBJECTIVE:To develop an HPLC method for the determination of 5-fluorouracil concentration in human plasma.METHODS:The concentration of 5-fluorouracil was assayed on a Ultimate C₁₈ column with a mobile phase consisting of 10mmolL^-1KH₂PO₄ at a flow rate of l.0mL.min^-1 and detected at 265nm.RESULTS:The calibration curve of 5-fluorouracil was linear over the range of 0.48μg.mL^-1～61.4μg.mL^-1 （r= 1.0000）.The low limit of determination was 0.48μg.mL^-1.The RSD of the intra-day and inter-day precisions were 5.12%⁹.98%and 5.28%～10.95%respectively.The samples were stable in room and low temperature,and were also stable in Free and thaw condition.CONCLUSION:This method is simple,precise,stabile and suit to be applied in the determination of 5-fluorouracil concentration and its Pharmacokinetic studies.更多还原