【作者】 黄月纯； 黄樱华； 刘翠玲； 陈慕媛； 魏刚； 刘东辉；

【机构】 广州中医药大学第一附属医院； 广州中医药大学；

【摘要】 目的研究AB-8型大孔树脂精制侧柏叶总黄酮的工艺条件。方法以总黄酮、槲皮苷含量结合指纹图谱主要特征峰面积保留率为考察指标;采用可见分光光度法测定总黄酮含量,HPLC法测定槲皮苷含量,HPLC法分析指纹图谱主要特征峰面积。结果 AB-8型大孔吸附树脂纯化效果较好,最佳工艺条件为:上样液浓度为含生药量0.20g·mL-1,上样量为每1mL湿树脂上样0.375g生药,吸附速率为1mL·min-1,洗脱液为4倍柱体积的70%乙醇,洗脱速率为2mL·min-1;树脂经95%乙醇和1moL·L-1氢氧化钠溶液再生后,可重复使用3次。总黄酮与槲皮苷的保留率达70%及95%以上,峰1～3的保留率达90%以上,纯化后总黄酮及槲皮苷的含量分别提高了2.48倍和3.29倍。结论 AB-8型大孔吸附树脂能较好地纯化富集侧柏叶总黄酮。更多还原

【Abstract】 Objective To study the process conditions of purifying the total flavonoids from Cacumen Platycladi by AB-8 resin.Methods Spectrophotometry method was used to determin the total flavonoids,and HPLC was used to determin quercitrin and analyze the characteristic peaks of Fingerprint.Results The purification effect was satisfactoryt when the concentration of original solution of the extract of Cacumen Platycladi was 0.20g·mL-1,the loading amount was 0.375g of Cacumen Platycladi per 1 mL of wet AB-8 resin,the adsorption velocity was 1 mL·min-1,the eluant was 70% alcohol with 4 times as much as the resin volume,and the elution velocity was 2 mL·min-1.AB-8 resin could be used for 3 times repeatedly after being reproducted by 95% of ethanol and 1 moL·L-1 of natrium hydroxydatum(NaOH).The remaining rate of the total flavonoids and quercitrin was over 70% and 95% respectively,and the remaining rate of peaks 1~3 was over 90%.After being purified by the AB-8 resin,the contents of the total flavonoids and quercitrin were raised 2.48 times and 3.29 times more than those before purification respectively.Conclusion AB-8 resin is fit for seperating and purifying the total flavonoids from Cacumen Platycladi.更多还原