【作者】 姚晖； 姜晓刚； 张继平； 焦河玲； 邓虹珠；

【机构】 佛山市第二人民医院药剂科； 山东省济宁医学院病理教研室； 南方医科大学中医药学院；

【摘要】 目的探讨哈蟆油对D-半乳糖所致雄性衰老大鼠肝脏组织信号传导与转录激活因子(Signal Transducer and Activator of Transcription,STAT3)蛋白表达的影响。方法 SPF级SD雄性大鼠18只,随机分为模型组、维生素E组(VE组)和哈蟆油组,每组6只,D-半乳糖颈背部皮下注射6周,另取雄性大鼠6只,同样部位每日注射生理盐水,作为空白组。第3周开始灌胃给药,给药时间30天。给药结束后收集标本,行HE染色观察病理变化;采用免疫组化法及Western-blotting法检测肝脏组织STAT3蛋白的表达变化。结果 HE染色表明模型组肝脏组织细胞体积增大,结构松散,衰老体征明确。服用哈蟆油能维持肝脏细胞形态,减轻衰老体征。免疫组化和Weatern-blotting结果显示,STAT3蛋白阳性细胞积分分别为8.25±0.50,.150±0.58,5.75±2.36,9.25±1.89;STAT3蛋白表达量(与内参比较)分别为27.39±1.13,20.26±0.88,21.26±1.17和30.15±1.03。模型组STAT3蛋白阳性细胞积分和蛋白表达量明显下降(P<0.01)。雄性衰老大鼠口服哈蟆油后,与模型组相比,哈蟆油组能明显上调肝脏STAT3蛋白的阳性细胞积分和蛋白表达量(P<0.01)。结论哈蟆油能上调STAT3蛋白表达,促进衰老雄性大鼠肝细胞增殖。哈蟆油延缓雄性衰老作用可能通过调控肝脏STAT3信号通路来促进有关增殖蛋白表达发挥抗衰老作用,与VE作用有所不同。更多还原

【Abstract】 Objective To investigate the expressions of liver tissue STAT3 in aged male rats with Oviductus Ranae(OR) by D-galactose.Methods 18 male SD rats(SPF grade) were randomly divided into 3 equal groups,namely the OR group,VE group and model group.The rats received subcutaneous injection of D-Galactose for 6 weeks to establish aging models.Another 6 rats were injected daily with normal saline (NS) to serve as the normal control group.From the third week of the experiment,the rats were given oral OR (in OR group) or Vitamin E (in VE group) till 30 days.After completion of drug administration,all the rats were sacrificed and analysis the changes of pathology in liver tissue by hematoxylin and erosin staining(HE),STAT3 were detected in these groups liver tissues by immunohistochemistry(IHC) and Western-boltting method.Results Cell morphologic and pathological structure liver tissue in aged rats was damaged.After taking OR,impairment of pathology was able to be restored and OR could maintain cellular normal form.Result of IHC showed positive cell integral were 8.25±0.50,.150±0.58,5.75±2.36,9.25±1.89,and Western-blotting showed expression of STAT3 protein were 27.39±1.13,20.26±0.88,21.26±1.17 and 30.15±1.03 (compare to β-actin).Compare with model group,positive cell integral and STAT3 expression of liver were obviously up-regulated in OR group(P<0.01).In contrast,model group was down-regulation(P<0.01).OR can enhance expression of STAT3 in liver tissue of male aged rats.These results indicate that OR delays senescence of organism in aged rats.Conclusions STAT3 expression may be up-regulated different level by OR.OR can enhance liver cell proliferation in aged male rats.OR may be up-regulation expression of aging proteins of liver to stay caducity.It is different from Vitamin E.更多还原