【作者】 郭艳丽； 孙凤； 袁洪林； 阎宏涛；

【Author】 GUO Yan-li~(1,2),SUN Feng~2,YUAN Hong-lin~1,YAN Hong-tao~2 1.State Key Laboratory of Continental Dynamics,Northwest University,Xi’an 710069,China 2.Key Laboratory of Synthetic and Natural Functional Molecule of Ministry of Education,College of Chemistry and Materials Science,Northwest University,Xi’an 710069,China

【机构】 西北大学大陆动力学教育部重点实验室； 西北大学合成与天然功能分子化学教育部重点实验室,西北大学化学与材料科学学院；

【摘要】 采用荧光光谱、吸收光谱和共振光散射光谱(RLS)研究了金纳米微粒(GNPS)分别与牛血清白蛋白(BSA)和人血清白蛋白(HSA)的相互作用。荧光光谱结果表明金纳米对血清蛋白的荧光具有猝灭作用,猝灭符合Stern-Volmer方程。讨论了金纳米微粒对血清蛋白荧光猝灭的机理,该荧光猝灭为动态猝灭。金纳米-血清蛋白体系的共振散射强度显著增大,且共振光散射信号的增强与蛋白质的含量呈线性关系,由此可期望建立一种金纳米为探针共振光散射测定蛋白质的新方法。更多还原

【Abstract】 The interactions of gold nanoparticles(GNPS) with bovine serum albumin(BSA) and human serum albumin(HSA) had been investigated by the fluorescence,UV-Vis absorption spectroscopy and resonance light scattering(RLS) spectroscopy approaches.The results showed that gold nanoparticles interacting with BSA and HSA’s fluorescence quenching were of a role in line fit into the Stern-Volmer quenching equation.The fluorescence quenching was the dynamic quenching.The system of resonance scattering intensity increased significantly,and resonance light-scattering signal enhanced with the protein content,and it had a linear relationship,which could look forward to establishing a new method to determine protein of gold nanoparticle as a probe by RLS.更多还原