【作者】 陈莹； 姚闽娜； 唐尧基； 李耀群；

【Author】 CHEN Ying,YAO Min-na,TANG Yao-ji,LI Yao-qun~* Department of Chemistry,the MOE Key Laboratory of Analytical Sciences,Xiamen University,Xiamen 361005,China

【机构】 厦门大学化学化工学院与现代分析科学教育部重点实验室；

【摘要】 建立了利用蛋白质吸附在液-固界面上的同步荧光信号来测定本体蛋白质溶液浓度的新方法。meson-四（4-对磺酸基苯基）卟啉（TPPS）标记的牛血清蛋白（BSA）在界面上的信号强度（同步荧光峰421 nm处）随本体溶液浓度的增加呈线性关系,检测限是94 ng·mL^-1。该法用于实际人血清蛋白样品的测定,结果令人满意。更多还原

【Abstract】 A new method of quantitative determination for serum albumin in aqueous solution has been developed by measuring the total internal reflection synchronous fluorescence at solid/liquid interface.The combination of bovine serum album（BSA） and meso-tetrakis （4-sulphonatophenyl） porphyrin（TPPS） adsorbed onto glass surface produced a synchronous fluorescence signal at 421 nm.At pH = 4.25,the signal intensity of BSA adsorbed on the interface was proportional to BSA concentration in bulk solution.The linear range of 1.0-8.0μg-mL and the detection limit of 0.93μg·mL^-1 were obtained.The human serum samples were determined with satisfactory results.更多还原