【作者】 赵雨； 陈心智； 曲晓波； 赵大庆；

【Author】 ZHAO Yu,CHEN Xin-zhi,QU Xiao-bo,ZHAO Da-qing~* Research and Development Center of New Medicine,Changchun College of Chinese Medicine,Changchun 130117,China

【机构】 长春中医学院新药研发中心；

【摘要】 应用拉曼光谱技术研究了稀土离子对DNA的影响。稀土离子Eu³⁺主要与DNA的PO₂发生结合,这种结合对脱氧核糖的振动产生影响,但对碱基的振动不产生影响。Ce³⁺首先通过断裂磷酸基团,进而影响碱基的堆砌,造成A-T间氢键断裂,B型构象消失,DNA双螺旋受破坏。并且由于核糖-磷酸键的断裂,使脱氧核糖的振动也发生改变。更多还原

【Abstract】 The structure of DNA will change by binding to mental ions.The effect of rare-earth ion on DNA is little investigated, especially the application of Raman spectroscopy in the field.The authors employ the Raman spectroscopy to study the interaction of rare earth with DNA and try to find binding site. Rare-earth ion Eu³⁺ could bind to phosphorous group and affect DNA structure on the deoxyribose but not the bases;Ce³⁺ hydrolyzes the PO₂^- group of DNA at first,and further effects the base stacking and results in the destroy of A-T rich region,the disappear of Bform conformation and deconstruction of DNA double-helix structure.Further,the vibration of ribose change due to the breakage of ribose -phosphorous bond.更多还原