【作者】 吴元菲； 吴德印； 任斌； 田中群；

【Author】 WU Yuan-fei,WU De-yin,REN Bin,TIAN Zhong-qun Dept.of Chem.and State Key Lab of Phys.Chem.of Solid Surfaces,Xiamen University,Xiamen 361005,China

【机构】 厦门大学化学系,固体表面物理化学国家重点实验室；

【摘要】 进行了DNA链(5’-AGC TCAGCAACGATG-3’)吸附在粗糙金电极和化学合成金纳米粒子上拉曼光谱的研究。对该DNA链的SERS谱峰进行了指认。结果表明粗糙金电极和金纳米粒子上的SERS谱有较大的差别。这主要是由于在两种SERS基底上DNA链的吸附构型不同。在12 nm金纳米粒子上,金纳米粒子与DNA链的碱基接触有利,而在金电极上DNA链主要以5’端A碱基吸附。这两种不同吸附方式导致SERS谱有较大的不同。更多还原

【Abstract】 This work presents a surface-enhanced Raman spectroscopic study of DNA(5-AGC TCAGCAACGATG-3’ ) adsorbed on a rough gold electrode and chemically synthesized gold nanoparticles.The SERS spectral bands are assigned.The SERS spectra from the rough gold electrode and gold nanoparticles show a large difference,which is interpreted by the different adsorption orientation of the DNA chain and the influence of the nanoparticle size.The DNA chain with aβconformer adsorbed on the rough gold electrode surface prefers an interaction through the 5’-end,so the Raman signal from the adenosine residue is significantly enhanced.While for the gold nanoparticles with a size of about 12 nm as the SERS substrate,all the four residues can interact with the nanoparticles,yielding observable SERS signals.更多还原