【作者】 姜兆林； 吴玉红； 谭家镒；

【Author】 Jiang zhao-lin Wu yu-hong Tan jia-yi

【机构】 中国刑警学院法化系；

【摘要】 <正>液液萃取一普通差示光谱法是测定生物检材中巴比妥类药物的常用方法,液液萃取及净化操作繁琐、冗长,应用不方便,普通差示光谱发易受杂质干扰灵敏度不高。固相萃取是较新的分离技术,具有操作简便、节省溶剂、萃取效率高等优点,导数光谱法是较新的光谱技术,具有抗杂质干扰能力强,灵敏度高等优点。我们经研究将两种新技术结合,建立了血和尿中国内常用巴比妥类药物含量的测定方法,该法操作简便、成本低廉,灵敏度较高、准确性较好。适于作为法医毒物分析的常规方法。更多还原

【Abstract】 A UV differential derivative spectrophotometry method for the determination of barbiturates,including phenobarbital,pentobarbital,amobarbital,secobarbital and barbital,in blood and urine after a simple and rapid solid-phase extraction procedure using GDX403 as sorbent is presented.In this method,the detection limits of barbiturates in blood and urine are about 1 ug/ml.For five replicates determination of 10 ug of barbiturates added to one millilitre of blood or urine,the relative standard deviations of recoveries are bellow 5%and the average recoveries are above 90%except for barbital,the average recoveries of which are a little more than 60%.更多还原