【作者】 刁波； 冯勇； 申元英； 熊海蓉； 罗凡； 杨占秋； 侯炜；

【Author】 Diao Bo1,Feng Yong1,Shen Yuan-ying2,Xiong Hai-rong1,Luo Fan1,Yang Zhan-qiu,Hou Wei1﹡.1.State Key Laboratory of Virology/Institute of Medical Virology,School of Basic Medical Sciences,Wuhan University,Wuhan 430071;2.College of Public Hygiene of Dali University.Dali 67100.

【机构】 病毒学国家重点实验室/武汉大学医学病毒学研究所； 大理学院公共卫生学院；

【摘要】 目的:探讨吸毒并HIV感染人群与HLA-DRB1基因多态性的相关性。方法:应用序列特异性引物聚合酶链反应(PCR-SSP)方法对41例无亲缘关系的吸毒合并HIV感染者及24例单纯吸毒者进行HLA-DRB1基因型检测,分析正常对照组和吸毒组及吸毒合并HIV感染组HLA-DRB1等位基因频率分布。结果:与正常对照组比较,吸毒组中,﹡0401-0411(P<0.05,RR=9.22),﹡1001(P<0.05,RR=19.27)的等位基因频率明显增高;在吸毒合并HIV感染组中,HLA-DRB1﹡0101-0103(P<0.01,RR=7.26)、﹡0401-0411(P<0.01,RR=16.73)、﹡0801-0806(P<0.01,RR=5.49)、﹡1001(P<0.05,RR=22.91)、﹡1401/1404/1405(P<0.05,RR=5.41)的等位基因频率显著增高,而DRB1﹡1501-1502(P<0.01,RR=0.18)、DRB5(P<0.01,RR=0.40)的等位基因频率较正常对照组明显减少。与吸毒组比较,吸毒合并HIV感染组中各等位基因频率变化无统计学差异。结论:HLA-DRB1等位基因动态性与吸毒合并HIV感染具有密切相关性,这对阐明疾病的发生机制及后期治疗和免疫预防具有重要的作用。更多还原

【Abstract】 Objective:To investigate the relationship between genetic polymorphism of HLA-DRB1 and Drug taking with HIV infection.Methods:A total of 41 drug users with HIV-1 infected patients and 24 drug users without blood relationship were selected and analyzed by using the polymerase chain reaction (PCR) with sequence-specific primers to test their HLA-DRB1 allele gene frequencies(GF),analyzing different distribution of HLA-DRB1 alleles in health control,drug user and Drug user with HIV infection patients.Results:Compared with health control group,a significantly higher frequency of DRB1 ﹡ 0401-0411(P<0.05,RR=9.22),﹡1001(P<0.05,RR=19.27) in drug user group;an obviously higher frequency of DRB1 ﹡ 0101-0103(P<0.01,RR=7.26),﹡ 0401-0411(P<0.01,RR=16.73),﹡ 0801-0806(P<0.01,RR=5.49),﹡1001(P<0.05,RR=22.91),﹡1401/1404/1405(P<0.05,RR=5.41) allele among drug user with HIV infected patients,in contract,the gene frequency of DRB1 ﹡1501-1502(P<0.01,RR=0.18),DRB5(P<0.01,RR=0.40) in healthy controls were significantly higher than in drug user with HIV infection patients.Compared with drug users group,the gene frequency of DRB1 was not statistically significant in drug user with HIV infection.Conclusion:There is close association between HLA-DRB1 alleles and drug users with HIV Infection,which plays a crucial role in interpreting the mechanism,therapeutics and prophylaxis in drug users with HIV infection.更多还原