【作者】 柯碧霞； 谭海玲； 邓小玲； 刘美真； 陈经雕； 钟豪杰； 李柏生； 柯昌文；

【Author】 KE Bi-xia,TANG Hai-ling,DENG Xiao-ling,LIU Mei-zheng,CHENG Jin-diao,ZHONG Hao-jie,LI Bo-sheng,KE Cang-wen.Guangdong Province Center for Disease Control and Prevention,Guangzhou 510300,China

【机构】 广东省疾病预防控制中心；

【摘要】 目的:了解广东省新型福氏志贺菌Fc菌株的流行与病原学特征。方法:对51株福氏志贺菌Fc菌株进行生化、血清学试验、药敏试验和PFGE分型;并用气相色谱仪测定其中6株的脂肪酸成分。结果:从2000～2004年广东省痢疾监测发现:福氏志贺菌2a的数量正逐年下降,而福氏志贺菌4c的数量却逐年上升;F4c的生化结果符合志贺菌特征,血清学均为福氏多价+,IV型因子+,7,8群血清+;F4c菌株对诺氟沙星、环丙沙星、头孢噻肟和头孢曲松四种药物敏感,对四环素、复方新诺明、萘啶酸、氯霉素和氨苄西林均产生耐药;PFGE对43株F4c菌株进行分子分型,共分为21个不同的型别;6株志贺菌F4c的主要脂肪酸成分为12∶0酸、14∶0酸、Sum In Feature2、Sum In Feature3、16∶0酸、17∶0cyclo、Sum In Feature8和19:0cyclo w8c等8种脂肪酸。结论:根据广东省志贺菌菌型的变化和福氏志贺菌4c的流行,提示F4c菌型已经适应了广东省生存环境并逐步演变成为一类新的稳定的福氏志贺菌的优势流行菌株。更多还原

【Abstract】 Objective:To understand the prevalence and nosazontology characterization of serologically atypical strains of shigella flexneri 4c isolated from guangdong province.Methods:51 F4c strains strains were tested by biochemistry, serum agglutination ,antibiotic susceptibility and PFGE; and determine the fatty acid constitutes of 6 Shigella flexneri strains using gas chromatograph technique. Results the biochemistry characteristics of these F4c strains were consistent with Shigella. F4c strains agglutinated with the serum of poly B,IV factor and Group 7 and 8..The results of 9 antibiotic susceptibility tests of 51 F4c strains demonstrated that they are only sensitive to norfloxacin , cefotaxime , ciprofloxacin and ceftriaxone.The drug resistance to tetracycline, trimesulf, nalidixan, and chloromycetin reached 100%, and ampicillin also reached 91.7%.43 F4c strains can be devided into 21 types by PFGE, Eight kinds of fatty acids were found; They are 12:0 acid, 14:0 acid, Sum In Feature 2, Sum In Feature 3, 16:0 acid, 17:0 cyclo, Sum In Feature 8 and 19:0 cyclo w8c, respectively. Conclusion:The changes of Shigellosis types and prevalence of F4c in Guang dong province indicate that F4c has already adapted to the habitat environment and is gradually emerging as a new dominant epidemic strain of Shigella flexneri.更多还原