【作者】 刘渠； 李涌； 陈应坚； 徐亚军；

【Author】 LIU qu,LI Yong,Chen Ying-jian etal.(Center for Disease Control and Prevention of LongGang, Shenzhen 518172,P.R.China.)~2

【机构】 深圳市龙岗区疾病预防控制中心； 四川大学华西公共卫生学院；

【摘要】 [目的]比较大肠菌群荧光定量PCR方法和多管发酵法检测结果的相关性。[方法]采用建立的大肠菌群荧光定量PCR方法和多管发酵法同时检测系列浓度梯度的实验室模拟水样的大肠菌群含量。对结果进行相关性分析,比较两种方法定量结果的相关性。[结果]大肠菌群荧光定量PCR能够能准确检测高于10~2CFU/100mL水平的水质大肠菌群含量,在此水平以上的定量结果同多管发酵法结果具有较高相关性。[结论]大肠菌群荧光定量PCR方法具有快速、特异、操作简便的特点,可以作为多管发酵法检测方法的补充,用于水质大肠菌群的快速定量检测。更多还原

【Abstract】 [Objective]To study the correlation between quantitative PCR and multiple tube fermentation method of coliform detection.[Methods]A series of simulated water samples were detected by fluorescence quantitative PCR established and multiple tube fermentation method simultaneously,the results were analysis to fred the correlation between the two methods.[Results] The fluorescence quantitative PCR of coliform could quantitative detect water samples which contained coliform higher than 10~2CFU/100mL.The result was significant correlated with the results of multiple fermentation method.[Conclusions]The fluorescence quantitative PCR method was a rapid,specific and convenient detection method of coliform,which could used as a complement of traditional method and apply to rapid quantitative detection in water.更多还原