【作者】 柯碧霞； 谭海玲； 李柏生； 何冬梅； 马聪； 刘美真； 陈经雕； 柯昌文；

【Author】 KE Bi-xia,TANG Hai-ling,LI Bo-sheng,HE Dong-mei,MA Chong, LUI Mei-zheng,CHEN Jin-diao,KE Cang-wen.Guangdong Province Center for Disease Control and Prevention,Guangzhou 510300,China

【机构】 广东省疾病预防控制中心；

【摘要】 目的了解2009年广东省副溶血弧菌食物中毒分离株和腹泻病人监测分离株的血清分型、毒力基因携带情况及分子特征。方法对2009年广东省副溶血弧菌食物中毒分离株95株和15株分离自腹泻病人监测的菌株进行血清分型,耐热直接溶血素相关基因(trh)和耐热直接溶血素基因(tdh)PCR检测以及选取不同血清型副溶血弧菌81株进行PFGE分子分型。结果2009年从腹泻病人监测中分离到15株副溶血弧菌,血清型以O3:K6(46.67%)和O4:K8(33.33%)为主,从11宗由副溶血弧菌污染引起的食物中毒分离到95株菌,血清型以O3:K6(44.21%)和O4:K8(28.42%)居多,7株食品分离株都不是O3:K6血清型;93株(84.54%)为tdh+trh-菌株,13株(11.81%)为tdh-trh-菌株,3株(3.65%)为tdh+trh+菌株;81株副溶血弧菌的PFGE相似值为57.7-100%,被分为36种不同的PFGE型别,PFGE001型和PFGE029型为2009年广东省副溶血弧菌优势PFGE型别。结论在广东省引起感染性腹泻和食物中毒的副溶血弧菌中O3:K6和O4:K8型是最主要的两种血清型,多数菌株携带tdh基因,存在优势PFGE型别菌株不断引起省内各地区的散发和暴发。更多还原

【Abstract】 Object To study the serotypes,virulence feactures and molecular characteristics of Vibrio parahaemolyticus isolated in food poisoning cases and surveillance of diarrhoea patents in Guangdong in 2009.Method 95 Vibrio parahaemolyticus strains from food poisoning cases and 15 strains from surveillance of diarrhoea patients were serotyped and detected tdh(thermostable direct hemolysin,tdh) and trh(tdh-related hemolysin gene,trh)by PCR.81 serovariant Vibrio parahaemolyticus strains were selected for PFGE subtyping. Results There were 15 Vibrio parahaemolyticus strains isolated from surveillance of diarrhoea patients and 95 strains were isolated from 11 Vibrio parahaemolyticus-caused food poisoning cases in 2009.Among these strains,O3:K6(46.67%and 44.21%) and O4:K8 (33.33%and 28.42%) were the dominant serotypes,but the 7 food-borne strains were not O3:K6.There were 93(84.54%) strains tdh+trh-,13(11.81%) strains tdh-trh-,and 3 (3.65%) strains tdh+trh+.The similarity value is 57.7%to 100%of the 81 strains after PFGE subtyping,and 36 PFGE subtypes were defined.PFGE001 and PFGE029 were the dominant subtypes.Conclusion O3:K6 and O4:K8 were the most dominant serotypes in Vibrio parahaemolyticus-caused diarrhoea and food poisoning cases in Guangdong.tdh were detected in most strains.The dominant PFGE subtypes are causing sporadic and outbreak cases in different areas within Guangdong.更多还原