【作者】 李文； 林育纯； 陈慧峰； 罗洁； 李晓杰； 林丽娜； 胡耀明； 张树江； 陈雯； 林忠宁；

【Author】 LI Wen,LIN Yuchun,CHEN Huifeng,LUO Jie,LI Xiaojie,LIN Lina,HU Yaoming,ZHANG Shujiang,CHEN Wen,LIN Zhongning~* (School of Public Health,Sun Yat-sen University,Guangzhou 510080,China)

【机构】 中山大学公共卫生学院；

【摘要】 目的探讨锌对镉化合物诱导人未成熟树突状细胞（iDC）毒性作用的影响。方法分离人外周血单核细胞,体外诱导获得iDC;分别给予生理盐水（对照组）,氯化镉（CdCl₂组）;硫酸锌（ZnSO₄组）;硫酸锌预处理后给予氯化镉（Zn+Cd组）;锌离子特异性螯合剂TPEN预处理后给予氯化镉（TPEN+Cd组）处理。用噻唑蓝颜色反应法（MTT）检测细胞活力;单细胞凝胶电泳（SCGE）检测细胞DNA单/双链断裂损伤（SSB/DSB）;吖啶橙（AO）与碘化丙啶（PI）双染色法检测细胞凋亡。结果与对照组相比,iDC活力随CdCl₂剂量增加明显下降,差别有统计学意义（p<0.05）;CdCl₂、Zn+Cd、TPEN+Cd三组与ZnSO₄组相比,在相同CdCl₂剂量时细胞活性均明显下降（p<0.05）;相同CdCl₂剂量（20μM）下,Zn+Cd和TPEN+Cd组细胞DNA损伤程度（尾长和尾矩）均明显增高（p<0.05）;各组细胞凋亡情况存在差别。结论镉化合物对诱导的人iDC有细胞毒性,且高于锌化合物;给予iDC硫酸锌（20μM）干预或耗竭细胞内锌离子增加氯化镉诱导iDC细胞DNA断裂损伤相关毒性的敏感性。更多还原

【Abstract】 Objective To study the effects of zinc compounds on the cytotoxicity of cadmium chloride to human immature dendritic cells（iDC） in vitro.Methods Cells were subgrouped: control（normal saline,NC）;zinc supplementation（pretreatment with zinc sulfate 12h before cadmium administration,Zn+Cd）;zinc depletion（pretreatment with TPEN 4h before cadmium administration,TPEN+Cd）;cadmium treatment（treated with varied concentration of cadmium chloride,CdCl₂）;zinc treatment（treated with varied concentration of zinc sulfate,ZnSO₄）.Cell viability was conducted by MTT assay;DNA brands breaks were detected with alkaline comet assay;cell apoptosis was observed through acridine orange（AO） and propidine iodide（PI） dyeing. Results Compared with NC,cell viability decreased significantly in a dose-response manner （p<0.05）;CdCl₂,Zn+Cd and TPEN+Cd treatment exerted greater effects on cell viability than ZnSO₄ did,at the same concentration（5、10、20、40、80、160μM）.DNA damage and cell apoptosis in Zn+Cd and TPEN+Cd group was much severe（p<0.05） and aggravated than 20μM CdCl₂ respectively.Conclution The cytotoxicity of cadmium chloride to iDC is stronger than zinc sulfate. Zinc supplementation（20μM） and depletion enhanced the cytotoxicity of cadmium chloride.更多还原