【作者】 陈建玲； 李文学； 杨光宇； 周志涛； 陈雯； 朱伟； 刘华章；

【Author】 CHEN Jian-ling~(1,2),LI Wen-xue~1,YANG Guang-yu~1,ZHOU Zhi-tao~3,CHEN Wen~2,ZHU Wei~(1*),LIU Hua-zhang~(1*) (1.Guangzhou Center for Diseases Control and Prevention,Guangzhou 510080;2.School of Public Health,Sun-sen University,Guangzhou 510080;3.School of Biotechnology,Southern Medical University Guangzhou 510515)

【机构】 广州市疾病预防控制中心； 中山大学公共卫生学院； 南方医科大学生物技术学院；

【摘要】 目的:为了解广州某茶叶市场湿仓储存普洱茶中真菌毒素的污染情况,对所抽取70份普洱茶样本中黄曲霉毒素（aflatoxin B1,AFB₁）、伏马毒素（fumonisin B1,FB₁）、呕吐毒素（脱氧雪腐镰刀菌烯醇,deoxynivalenol,DON）和T-2毒素的浓度水平进行检测,旨在为制定有关普洱茶叶安全卫生管理规范和相关的卫生标准提供数据资料。方法:随机抽取广州某茶叶市场湿仓储存普洱茶70份,破碎、浸提、过滤后采用免疫亲和层析净化-高效液相色谱法检测AFB₁,ELISA试剂盒检测FB₁、DON和T-2毒素的污染情况。结果:本次调查所采70份普洱茶样品中,AFB₁污染水平超出标准规定限值（5 g/kg）的有8/70（11.43%）例,DON污染水平超出标准规定限值（1 mg/kg）的有63/70（90%）例,FB₁和T-2毒素污染水平均未超出标准规定限值（分别为1 mg/kg和100 g/kg）。结论:本调查结果提示广州市该茶叶市场中的湿仓储存普洱茶存在不同程度的AFB₁及DON污染情况,而FB₁及T-2毒素虽未超出标准规定的限值,但在样品中均可检出,应引起广泛重视。更多还原

【Abstract】 OBJECTIVE:To understand the contaminating levels of bioligical toxins including aflatoxinB1（AFB₁）,FumonisinB1（FB₁）,DON and T-2 toxins of Pu’er tea in a tea market of Guangzhou,China, designing to provide useful information and data for safety and health management and related health standards. METHODS:Sample 70 Pu’er tea randomizely in a tea market of Guangzhou.All the samples were assayed with ELISA kit for FB₁,DON,T-2 toxin,and with Immunoaffinity chromatography-high performance liquid chromatography for AFB₁.RESULTS:The survey collected 70 samples of tea,the AFB₁ levels of 8 samples are above the limit value of national standard（5 g/kg）,and the DON levels of 63 samples are above the limit value of national standard（1mg/kg）,while the levels of FB₁ and T-2 toxin are below the limit standards（1mg/kg and 100 g/kg,respectively）.CONCLUSION:The survey results indicated that AFB1 and DON in the samples are higher and exceed the limit standares to some degree.Althouth the levels of FB1 and T-2 are below,the existence of them in Pu’er tea should be paid close attention to.更多还原