【作者】 郝春英； 孟庆芳； 赤国彤； 刘大群；

【Author】 Hao Chunying,Meng Qingfang,Chi Guotong,Liu Daqun (College of Plant Protection,Agriculture University of Hebei,Biological Control Centre of Plant Pathogens and Plant Pests of Hebei Province,Baoding,071001)

【机构】 河北农业大学植物保护学院河北省农作物病虫害生物防治工程技术研究中心；

【摘要】 研究了发酵时间、初始pH值、外加碳源、氮源及无机盐对其产孢量的影响。结果表明,产孢的最适培养时间为10d;最佳初始pH值为自然pH值;添加葡萄糖和蛋白胨可促进链霉菌Men-myco-93-63产孢,其适宜的添加量分别为1.0%和2.0%;添加适量的钙盐可进一步提高产孢量。在上述条件下培养,产孢量最高可达到4.85×1 0~9cfu/g。更多还原

【Abstract】 In order to enhance sporulation of Streptomyces Men-myco-93-63,the influencing factors on its sporulation were investigated,including fermentation time,initial pH,carbon source,nitrogen source and inorganic.Results suggested that optimal fermentation time for sporulation was 10d,optimal pH was unartificial,glucose and peptone were demonstrated to be more suitable for sporulation of Streptomyces Men-myco-93-63,and these condign dosage was 1.0% and 2.0%,Ca²⁺could enhance sporulation of Streptomyces Men-myco-93-63.In this culture conditions,the highest sporulation was 4.85×10⁹ cfu/g.更多还原