【作者】 陈洁； 吕志亮； 贾丽；

【Author】 CHEN Jie,L Zhi-liang,JIA Li (Ministry of Education Key Laboratory of Laser Life Science & Institute of Laser Life Science, South China Normal University,Guangzhou 510631,China)

【机构】 华南师范大学激光生命科学教育部重点实验室&激光生命科学研究所；

【摘要】 该文首次报道了以碳二亚胺作为催化剂,基于羧甲基壳聚糖(CMCH)化学修饰的硅胶整体柱的制备。壳聚糖上的氨基和羟基为色谱分离提供了弱阴离子交换/亲水作用的作用位点。通过分离极性酸性化合物对所制备的壳聚糖修饰硅胶整体柱进行评价,该整体柱对核苷酸、核苷与碱基、有机酸等极性化合物具有良好的分离选择性。对分离机理的研究结果显示,壳聚糖修饰硅胶整体柱对这些化合物的分离主要基于亲水相互作用。更多还原

【Abstract】 A novel cationic hydrophilic interaction monolithic stationary phase based on the chemical modification of carboxymethyl chitosan(CMCH)to the monolithic silica skeleton using carbodiimide as an activation reagent was prepared for performing capillary liquid chromatography.The amino and hydroxyl moieties of CMCH functioned as both the ion-exchange sites and polar providers.The performance of the column was studied by the separation of polar acidic compounds.The chitosan functionalized monolithic silica column showed good selectivity for nucleosides,nucleotides,aromatic acids and aliphatic acids.The mechanism for the separation of these compounds was also studied. The results showed that these compounds were separated primarily based on the hydrophilic interaction mechanism.更多还原