【作者】 麦智彬； 戴宗； 邹小勇；

【Author】 MAI Zhi-bin,DAI Zong,ZOU Xiao-yong (School of Chemistry and Chemical Engineering,Sun Yat-sen University,Guangzhou 510275,China)

【机构】 中山大学化学与化学工程学院；

【摘要】 利用表面等离子体共振（SPR）技术研究了pH诱导下血红蛋白的构象变化规律。分别以碱→酸及酸→碱两种pH值梯度动态改变传感界面固定化血红蛋白的构象。结果表明,当pH从碱→酸变化,表观SPR(Δθ_app)在碱性条件下变化较快,在酸性条件下变化较慢;pH从酸→碱变化时,Δθ_app在pH<5.10时迅速减小,在pH 5.10～6.10时出现平台,在pH 6.10～8.38时再次迅速减小,在pH>8.38时变化缓慢。两种诱导作用产生的Δθ_app信号差异可能由于血红蛋白在酸性条件下亚单位分裂引起表面电荷变化,进而使传感界面的电荷及介电常数变化所致。更多还原

【Abstract】 Surface plasmon resonance（SPR） was used to characterize the conformational changes of Hb induced by different pHs.When pHs varied from acidic condition to the basic condition,Δθ_app decreased in concomitance with the decrease of the pHs,as well.When pH was below 5.10,the changing rate ofΔθ_app was larger than that of the basic condition.And a plateau region was found between pH 5.10 and pH 6.10.When pH changed from 6.10 to 8.38,the resultingΔθ_app of Hb showed significant decrease.As pHs were larger than 8.38,the increasing rate ofΔθwas slowed down.The apparent discrepancy was ascribed to the subunit dissociation of Hb,which would alter the surface charges and dielectric constant of the Hb layer.更多还原