【作者】 王飞； 农云军； 苏流坤； 窦文渊；

【Author】 WANG Fei,NONG Yun-jun,SU Liu-kun,DOU Wen-yuan (Guangdong Key Laboratory of Emergency Chemical Test,China National Analytical Center(Guangzhou), Guangzhou 510070,China)

【机构】 中国广州分析测试中心广东省化学危害应急检测技术重点实验室；

【摘要】 建立了同时测定人参中的12种人参皂苷单体的HPLC-ELSD分析方法。以常规C₁₈柱作为色谱分离柱,以甲醇-水-醋酸铵为流动相,通过优化梯度洗脱条件,成功实现了12种人参单体皂苷R_g1、R_g2、R_g3、R_e、R_f、R_h1、R_h2、R_b1、R_b2、R_b3、R_c、R_d的有效分离,分离度均大于1.0。各单体皂苷色谱峰面积的自然对数与浓度的自然对数呈良好的线性关系（r>0.991）,检出限为44～253 ng,样品的加标回收率为88%～97%（n=6）,相对标准偏差小于4.0%。更多还原

【Abstract】 A HPLC - ELSD method was established for the determination of twelve ginsenoides,including R_g1,R_g2,R_g3,R_e,R_f,R₍h1_,R_h2,R_b1,R_b2,R_b3,R_c and R_d in panax ginseng.The ginsenoides wer successfully separated on a ODS-C₁₈ column（4.6 mm×250 mm,5μm） using methanol - water - ammonium acetate as mobile phase with gradient elution.The resolution of twelve ginsenoides were more than 1.0.there was a good linear relationship（r>0.991） between the chromatographic area and the concentration of the monomer ginsenoides.The detection limits were in the range of 44 -253 ng.The spiked recoveries（n=6） were in the range of 88%-97%with relative standard deviations less than 4.0%.更多还原